Examining histone modification crosstalk using immobilized libraries established from ligation-ready nucleosomes

Files
d0sc03407j.pdf (1.05 MB)

License
http://creativecommons.org/licenses/by-nc/3.0/

Type
Article

Authors
Aparicio Pelaz, Diego
Yerkesh, Zhadyra
Kirchgäßner, Sören
Mahler, Henriette
Kharchenko, Vladlena
Azhibek, Dulat
Jaremko, Mariusz
Mootz, Henning D.
Jaremko, Lukasz
Schwarzer, Dirk
Fischle, Wolfgang

KAUST Department
Bioscience Program
Biological and Environmental Sciences and Engineering (BESE) Division
Bioscience

Date
2020

Submitted Date
2020-06-19

Abstract
280 different patterns of histone modifications were installed in preassembled nucleosomes using PTS and SML enabling screening of readout crosstalk.

Citation
Aparicio Pelaz, D., Yerkesh, Z., Kirchgäßner, S., Mahler, H., Kharchenko, V., Azhibek, D., … Fischle, W. (2020). Examining histone modification crosstalk using immobilized libraries established from ligation-ready nucleosomes. Chemical Science. doi:10.1039/d0sc03407j

Acknowledgements
This work was supported by the priority program SPP 1623 of the Deutsche Forschungsgemeinscha (DFG FI1513/3-1 to W. F. and SCHW1163/4-1 to D. S.) the Max Planck Society (W. F.), and intramural funds of King Abdullah University of Science and Technology (M. J., L. J. and W. F.). We thank members of the HDM group for help with establishing protein trans-splicing assays.

Publisher
Royal Society of Chemistry (RSC)

Journal
Chemical Science

DOI
10.1039/d0sc03407j

Additional Links
http://xlink.rsc.org/?DOI=D0SC03407J

Permanent link to this record
http://hdl.handle.net/10754/664743
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Biological and Environmental Science and Engineering (BESE) Division
Bioscience Program