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dc.contributor.authorDuarte, Carlos M.
dc.contributor.authorNgugi, David
dc.contributor.authorAlam, Intikhab
dc.contributor.authorPearman, John K.
dc.contributor.authorKamau, Allan
dc.contributor.authorEguíluz, V. M.
dc.contributor.authorGojobori, Takashi
dc.contributor.authorAcinas, Silvia G.
dc.contributor.authorGasol, Josep M.
dc.contributor.authorBajic, Vladimir B.
dc.contributor.authorIrigoien, Xabier
dc.date.accessioned2020-08-11T06:11:58Z
dc.date.available2020-08-11T06:11:58Z
dc.date.issued2020-09-29
dc.date.submitted2020-03-14
dc.identifier.citationDuarte, C. M., Ngugi, D. K., Alam, I., Pearman, J., Kamau, A., Eguiluz, V. M., … Irigoien, X. (2020). Sequencing Effort Dictates Gene Discovery in Marine Microbial Metagenomes. Environmental Microbiology. doi:10.1111/1462-2920.15182
dc.identifier.issn1462-2912
dc.identifier.issn1462-2920
dc.identifier.pmid32743860
dc.identifier.doi10.1111/1462-2920.15182
dc.identifier.urihttp://hdl.handle.net/10754/664543
dc.description.abstractMassive metagenomic sequencing combined with gene prediction methods were previously used to compile the gene catalogue of ocean and host-associated microbes. Global expeditions conducted over the past 15 years have sampled to ocean to build a catalogue of genes from pelagic microbes. Here we undertook a large sequencing effort of a perturbed Red Sea plankton community to uncover that the rate of gene discovery increases continuously with sequencing effort, with no indication that the retrieved 2.83 million non-redundant (complete) genes predicted from the experiment represented a nearly complete inventory of the genes present in the sampled community (i.e. no evidence of saturation). The underlying reason is the Pareto-like distribution of the abundance of genes in the plankton community, resulting in a very long tail of millions of genes present at remarkably low abundances, which can only be retrieved through massive sequencing. Microbial metagenomic projects retrieve variable number of unique genes per Tera base-pair (Tbp), with a median value of 14.7 million unique genes per Tbp sequenced across projects. The increase in the rate of gene discovery in microbial metagenomes with sequencing effort implies that there is ample room for new gene discovery in further ocean and holobiont sequencing studies This article is protected by copyright. All rights reserved.
dc.publisherWiley
dc.relation.urlhttps://onlinelibrary.wiley.com/doi/abs/10.1111/1462-2920.15182
dc.rightsArchived with thanks to Environmental Microbiology
dc.titleSequencing Effort Dictates Gene Discovery in Marine Microbial Metagenomes
dc.typeArticle
dc.contributor.departmentApplied Mathematics and Computational Science Program
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
dc.contributor.departmentMarine Science Program
dc.contributor.departmentPlankton ecology Research Group
dc.contributor.departmentRed Sea Research Center (RSRC)
dc.identifier.journalEnvironmental Microbiology
dc.rights.embargodate2021-08-03
dc.eprint.versionPost-print
dc.contributor.institutionLeibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7B 38124 Braunschweig Germany
dc.contributor.institutionInstituto de Física Interdisciplinar y Sistemas Complejos IFISC (CSIC-UIB), E07122 Palma de Mallorca Spain
dc.contributor.institutionInstitut de Ciències del Mar, CSIC Barcelona Spain
dc.contributor.institutionCenter for Marine Ecosystems ResearchEdith Cowan University Joondalup Australia
dc.contributor.institutionAZTI – Marine Research, Herrera Kaia, Portualdea z/g, 20110 Pasaia (Gipuzkoa) Spain
kaust.personDuarte, Carlos M.
kaust.personNgugi, David
kaust.personAlam, Intikhab
kaust.personPearman, John K.
kaust.personKamau, Allan
kaust.personGojobori, Takashi
kaust.personBajic, Vladimir B.
kaust.personIrigoien, Xabier
dc.date.accepted2020-07-31
refterms.dateFOA2020-08-11T06:13:32Z
dc.date.published-online2020-09-29
dc.date.published-print2020-11


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