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dc.contributor.authorBaier, Thomas
dc.contributor.authorJacobebbinghaus, Nick
dc.contributor.authorEinhaus, Alexander
dc.contributor.authorLauersen, Kyle J.
dc.contributor.authorKruse, Olaf
dc.date.accessioned2020-08-09T13:03:15Z
dc.date.available2020-08-09T13:03:15Z
dc.date.issued2020-07-30
dc.date.submitted2020-04-17
dc.identifier.citationBaier, T., Jacobebbinghaus, N., Einhaus, A., Lauersen, K. J., & Kruse, O. (2020). Introns mediate post-transcriptional enhancement of nuclear gene expression in the green microalga Chlamydomonas reinhardtii. PLOS Genetics, 16(7), e1008944. doi:10.1371/journal.pgen.1008944
dc.identifier.issn1553-7404
dc.identifier.pmid32730252
dc.identifier.doi10.1371/journal.pgen.1008944
dc.identifier.urihttp://hdl.handle.net/10754/664526
dc.description.abstractEfficient nuclear transgene expression in the green microalga Chlamydomonas reinhardtii is generally hindered by low transcription rates. Introns can increase transcript abundance by a process called Intron-Mediated Enhancement (IME) in this alga and has been broadly observed in other eukaryotes. However, the mechanisms of IME in microalgae are poorly understood. Here, we identified 33 native introns from highly expressed genes in C. reinhardtii selected from transcriptome studies as well as 13 non-native introns. We investigated their IME capacities and probed the mechanism of action by modification of splice sites, internal sequence motifs, and position within transgenes. Several introns were found to elicit strong IME and found to be broadly applicable in different expression constructs. We determined that IME in C. reinhardtii exclusively occurs from introns within transcribed ORFs regardless of the promoter and is not induced by traditional enhancers of transcription. Our results elucidate some mechanistic details of IME in C. reinhardtii, which are similar to those observed in higher plants yet underly distinctly different induction processes. Our findings narrow the focus of targets responsible for algal IME and provides evidence that introns are underestimated regulators of C. reinhardtii nuclear gene expression.
dc.description.sponsorshipThe authors would like to express thanks to Prof. Dr. Alan Rose for sharing the introns COR15a and UBQ10 and to Prof. Dr. Ralph Bock for sharing the strain UVM4.
dc.publisherPublic Library of Science (PLoS)
dc.relation.urlhttps://dx.plos.org/10.1371/journal.pgen.1008944
dc.rightsThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleIntrons mediate post-transcriptional enhancement of nuclear gene expression in the green microalga Chlamydomonas reinhardtii
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal, Kingdom of Saudi Arabia.
dc.identifier.journalPLOS Genetics
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionBielefeld University, Faculty of Biology, Center for Biotechnology (CeBiTec), Universita¨tsstrasse, Bielefeld, Germany.
dc.identifier.volume16
dc.identifier.issue7
dc.identifier.pagese1008944
kaust.personLauersen, Kyle J.
dc.date.accepted2020-06-19
refterms.dateFOA2020-08-09T13:04:44Z


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This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Except where otherwise noted, this item's license is described as This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.