Comparative Genome-Centric Analysis of Freshwater and Marine ANAMMOX Cultures Suggests Functional Redundancy in Nitrogen Removal Processes
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Environmental Biotechnology Research Group
Environmental Science and Engineering Program
Water Desalination and Reuse Center (WDRC), Biological and Environmental Science Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia
Water Desalination and Reuse Research Center (WDRC)
KAUST Grant NumberFCC/1/ 1971-05-01
Permanent link to this recordhttp://hdl.handle.net/10754/664524
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AbstractThere is a lack of understanding of the interaction between anammox bacteria and the flanking microbial communities in both freshwater (non-saline) and marine (saline) ecosystems. Here, we present a comparative genome-based exploration of two different anammox bioreactors, through the analysis of 23 metagenome-assembled genomes (MAGs), 12 from freshwater anammox reactor (FWR), and 11 from marine anammox reactor (MWR). To understand the contribution of individual members to community functions, we applied the index of replication (iRep) to determine bacteria that are actively replicating. Using genomic content and iRep information, we provided a potential ecological role for the dominant members of the community based on the reactor operating conditions. In the non-saline system, anammox (Candidatus Brocadia sinica) and auxotrophic neighboring bacteria belonging to the phyla Ignavibacteriae and Chloroflexi might interact to reduce nitrate to nitrite for direct use by anammox bacteria. Whereas, in the saline reactor, anammox bacterium (Ca. Scalindua erythraensis) and flanking community belonging to phyla Planctomycetes (different than anammox bacteria)—which persistently growing in the system—may catabolize detritus and extracellular material and recycle nitrate to nitrite for direct use by anammox bacteria. Despite different microbial communities, there was functional redundancy in both ecosystems. These results signify the potential application of marine anammox bacteria for treating saline N-rich wastewaters.
CitationAli, M., Shaw, D. R., Albertsen, M., & Saikaly, P. E. (2020). Comparative Genome-Centric Analysis of Freshwater and Marine ANAMMOX Cultures Suggests Functional Redundancy in Nitrogen Removal Processes. Frontiers in Microbiology, 11. doi:10.3389/fmicb.2020.01637
SponsorsFunding. This work was supported by Center Competitive Funding Program (FCC/1/ 1971-05-01) from King Abdullah University of Science and Technology (KAUST). MAlb was supported by a research grant (15510) from VILLUM FONDEN.
PublisherFrontiers Media SA
JournalFrontiers in Microbiology
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