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dc.contributor.authorJiwani, Shahwar Imran
dc.contributor.authorGillis, Richard B
dc.contributor.authorBesong, David
dc.contributor.authorAlmutairi, Fahad
dc.contributor.authorErten, Tayyibe
dc.contributor.authorKök, M Samil
dc.contributor.authorHarding, Stephen E
dc.contributor.authorPaulsen, Berit S
dc.contributor.authorAdams, Gary G
dc.date.accessioned2020-08-09T10:06:14Z
dc.date.available2020-08-09T10:06:14Z
dc.date.issued2020-07-24
dc.date.submitted2020-06-19
dc.identifier.citationJiwani, S. I., Gillis, R. B., Besong, D., Almutairi, F., Erten, T., Kök, M. S., … Adams, G. G. (2020). Isolation and Biophysical Characterisation of Bioactive Polysaccharides from Cucurbita Moschata (Butternut Squash). Polymers, 12(8), 1650. doi:10.3390/polym12081650
dc.identifier.issn2073-4360
dc.identifier.pmid32722155
dc.identifier.doi10.3390/polym12081650
dc.identifier.urihttp://hdl.handle.net/10754/664517
dc.description.abstractCucurbits are plants that have been used frequently as functional foods. This study includes the extraction, isolation, and characterisation of the mesocarp polysaccharide of Cucurbita moschata. The polysaccharide component was purified by gel filtration into three fractions (NJBTF1, NJBTF2, and NJBTF3) of different molecular weights. Characterisation includes the hydrodynamic properties, identification of monosaccharide composition, and bioactivity. Sedimentation velocity also indicated the presence of small amounts of additional discrete higher molecular weight components even after fractionation. Sedimentation equilibrium revealed respective weight average molecular weights of 90, 31, and 19 kDa, with the higher fractions (NJBTF1 and NJBTF2) indicating a tendency to self-associate. Based on the limited amount of data (combinations of 3 sets of viscosity and sedimentation data corresponding to the 3 fractions), HYDFIT indicates an extended, semi-flexible coil conformation. Of all the fractions obtained, NJBTF1 showed the highest bioactivity. All fractions contained galacturonic acid and variable amounts of neutral sugars. To probe further, the extent of glycosidic linkages in NJBTF1 was estimated using gas chromatography-mass spectrometry (GCMS), yielding a high galacturonic acid content (for pectin polysaccharide) and the presence of fructans-the first evidence of fructans (levan) in the mesocarp. Our understanding of the size and structural flexibility together with the high bioactivity suggests that the polysaccharide obtained from C. moschata has the potential to be developed into a therapeutic agent.
dc.publisherMDPI AG
dc.relation.urlhttps://www.mdpi.com/2073-4360/12/8/1650
dc.relation.urlhttps://www.mdpi.com/2073-4360/12/8/1650/pdf
dc.rightsThis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleIsolation and Biophysical Characterisation of Bioactive Polysaccharides from Cucurbita Moschata (Butternut Squash).
dc.typeArticle
dc.contributor.departmentSolar and Photovoltaics Engineering Center, King Abdullah University of Science and Technology, Thuwal, Makkah 23955-6900, Saudi Arabia.
dc.identifier.journalPolymers
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionQueen’s Medical Centre, Faculty of Medicine and Health Sciences, University of Nottingham, Clifton Boulevard, Nottingham NG7 2UH, UK.
dc.contributor.institutionDepartment of Biochemistry, Faculty of Science, University of Tabuk, P.O. Box 741, Tabuk 71491, Saudi Arabia.
dc.contributor.institutionDepartment of Nutrition and Dietetics, Faculty of Health Sciences, Bayburt University, 69000 Baybur Turkey.
dc.contributor.institutionDepartment of Food Engineering, Faculty of Engineering & Architecture, Abant Izzet Baysal University, Gölköy, 14300 Bolu, Turkey.
dc.contributor.institutionNational Centre for Macromolecular Hydrodynamics (NCMH), School of Biosciences, Sutton Bonington Campus, The University of Nottingham, Sutton Bonington, Leicestershire LE12 5RD, UK.
dc.contributor.institutionDepartment of Pharmaceutical Chemistry, School of Pharmacy, Section Pharmacognosy, University of Oslo, PB 1068, Blindern, N-0316 Oslo, Norway.
dc.identifier.volume12
dc.identifier.issue8
dc.identifier.pages1650
kaust.personBesong, David
dc.date.accepted2020-07-22
refterms.dateFOA2020-08-09T10:06:53Z


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