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dc.contributor.authorMourier, Tobias
dc.contributor.authorde Alvarenga, Denise Anete Madureira
dc.contributor.authorKaushik, Abhinav
dc.contributor.authorde Pina-Costa, Anielle
dc.contributor.authorDouvropoulou, Olga
dc.contributor.authorGuan, Qingtian
dc.contributor.authorGuzmán-Vega, Francisco J.
dc.contributor.authorForrester, Sarah
dc.contributor.authorde Abreu, Filipe Vieira Santos
dc.contributor.authorJúnior, Cesare Bianco
dc.contributor.authorJunior, Julio Cesar de Souza
dc.contributor.authorMoreira, Silvia Bahadian
dc.contributor.authorHirano, Zelinda Maria Braga
dc.contributor.authorPissinatti, Alcides
dc.contributor.authorFerreira-da-Cruz, Maria de Fátima
dc.contributor.authorde Oliveira, Ricardo Lourenço
dc.contributor.authorArold, Stefan T.
dc.contributor.authorJeffares, Daniel C.
dc.contributor.authorBrasil, Patrícia
dc.contributor.authorde Brito, Cristiana Ferreira Alves
dc.contributor.authorCulleton, Richard
dc.contributor.authorDaniel-Ribeiro, Cláudio Tadeu
dc.contributor.authorPain, Arnab
dc.identifier.citationMourier, T., de Alvarenga, D. A. M., Kaushik, A., de Pina-Costa, A., Douvropoulou, O., Guan, Q., … Pain, A. (2019). The genome of the zoonotic malaria parasite Plasmodium simium reveals adaptations to host-switching. doi:10.1101/841171
dc.description.abstractSummaryPlasmodium simium, a malaria parasite of non-human primates in the Atlantic forest region of Brazil was recently shown to cause zoonotic infections in humans. Phylogenetic analyses based on the whole genome sequences of six P. simium isolates from humans and two isolates from brown howler monkeys revealed that P. simium is monophyletic within the broader diversity of South American Plasmodium vivax, consistent with the hypothesis that P. simium first infected non-human primates as a result of a host-switch of P. vivax from humans. Very low levels of genetic diversity within P. simium and the absence of P. simium-P. vivax hybrids suggest that the P. simium population emerged recently with a subsequent period of independent evolution in Platyrrhini monkeys. We find that Plasmodium Interspersed Repeat (PIR) genes, Plasmodium Helical Interspersed Subtelomeric (PHIST) genes and Tryptophan-Rich Antigen (TRAg) genes in P. simium are divergent from P. vivax orthologues and are enriched for non-synonymous single nucleotide polymorphisms, consistent with the rapid evolution of these genes. Analysis of genes involved in erythrocyte invasion revealed several notable differences between P. vivax and P. simium, including large deletions within the coding region of the Duffy Binding Protein 1 (DBP1) and Reticulocyte Binding Protein 2a (RBP2a) genes of P. simium. Sequence analysis of P. simium isolates from non-human primates (NHPs) and zoonotic human infections revealed a deletion of 38 amino acids in DBP1 present in all human-derived isolates, whereas NHP isolates were multi-allelic at this locus. We speculate that these deletions in key erythrocyte invasion ligands along with other significant genetic changes may have facilitated zoonotic transfer to humans. NHPs are a reservoir of parasites potentially infectious to humans that must be considered in malaria eradication efforts. The P. simium genome is an important resource for understanding the mechanisms of malaria parasite zoonoses.
dc.description.sponsorshipThe work was supported financially by the King Abdullah University of Science and Technology (KAUST) through the baseline fund BRF1020/01/01 to AP and BAS/1/1056-01-01 to STA, and the Award No. URF/1/1976-25 from the Office of Sponsored Research (OSR). The field work in the Atlantic Forest and laboratory analysis in Brazil received financial support from the Secretary for Health Surveillance of the Ministry of Health through the Global Fund (agreement IOC-005-Fio-13), Programa Nacional de Excelência (PRONEX) and contract 407873/2018-0 of the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), the Fundação de Amparo à Pesquisa do Estado de Minas Gerais (Fapemig CBB-APQ-02620-15) and the Fundação Carlos Chagas Filho de Amparo Pesquisa do Estado do Rio de Janeiro (Faperj), Brazil. CNPq supports CFAB, CTDR,MFFC, PB and RLO, with a research productivity fellowship. CTDR (CNE: E-26/202.921/2018), MFFC, PB and RLO are also supported by Faperj as Cientistas do nosso estado. AdP-C was supported by a postdoctoral fellowship from the Faperj and DAMA by a fellowship from the CGZV-SVS (Brazilian Ministry of Health) TED 49/2018 grant. SF was supported by a Wellcome Seed Award in Science to DCJ (208965/Z/17/Z).
dc.publisherCold Spring Harbor Laboratory
dc.rightsArchived with thanks to Cold Spring Harbor Laboratory
dc.titleThe genome of the zoonotic malaria parasite Plasmodium simium reveals adaptations to host-switching
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
dc.contributor.departmentPathogen Genomics Laboratory
dc.contributor.departmentStructural Biology and Engineering
dc.contributor.institutionGrupo de Pesquisa em Biologia Molecular e Imunologia da Malária, Fundação Oswaldo Cruz (Fiocruz), Belo Horizonte/MG, 30190-009, Brazil.
dc.contributor.institutionCentro de Pesquisa, Diagnóstico e Treinamento em Malária (CPD-Mal), Fiocruz, Rio de Janeiro/RJ, 21040-360, Brazil.
dc.contributor.institutionLaboratório de Pesquisa Clínica em Doenças Febris Agudas, Instituto Nacional de Infectologia Evandro Chagas, Fiocruz, Rio de Janeiro/RJ, 21040-360, Brazil.
dc.contributor.institutionCentro Universitário Serra dos Órgãos (UNIFESO), Teresópolis/RJ, 25964-004, Brazil.
dc.contributor.institutionLaboratório de Mosquitos Transmissores de Hematozoários. Instituto Oswaldo Cruz (IOC), Fiocruz, Rio de Janeiro/RJ, 21040-360, Brazil.
dc.contributor.institutionDepartment of Biology and York Biomedical Research Institute, University of York, Wentworth Way, York, YO10 5DD, U.K.
dc.contributor.institutionLaboratório de Pesquisa em Malária, IOC, Fiocruz, Rio de Janeiro/RJ, 21040-360, Brazil.
dc.contributor.institutionUniversidade Regional de Blumenau (FURB), Centro de Pesquisas Biológicas de Indaial (CEPESBI)/ Projeto bugio, Blumenau and Indaial, SC, Brazil.
dc.contributor.institutionCentro de Primatologia do Rio de Janeiro (CPRJ/Inea), 25940-000, Guapimirim, RJ, Brazil.
dc.contributor.institutionCentre de Biochimie Structurale, CNRS, INSERM, Université de Montpellier, 34090 35 Montpellier, France.
dc.contributor.institutionMalaria Unit, Department of Pathology, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan.
dc.contributor.institutionGlobal Station for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, N20 W10 Kita-ku, Sapporo, Japan.
dc.contributor.institutionNuffield Division of Clinical Laboratory Sciences (NDCLS), University of Oxford, Headington, Oxford, OX3 9DU, UK.
kaust.personMourier, Tobias
kaust.personKaushik, Abhinav
kaust.personDouvropoulou, Olga
kaust.personGuan, Qingtian
kaust.personGuzmán-Vega, Francisco J.
kaust.personArold, Stefan T.
kaust.personPain, Arnab
kaust.acknowledged.supportUnitOffice of Sponsored Research (OSR)

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