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dc.contributor.authorBertoncini, Andrea
dc.contributor.authorLaptenok, Siarhei
dc.contributor.authorGenchi, Luca
dc.contributor.authorRajamanickam, Vijayakumar Palanisamy
dc.contributor.authorLiberale, Carlo
dc.date.accessioned2020-07-14T10:02:03Z
dc.date.available2020-07-14T10:02:03Z
dc.date.issued2020-08-10
dc.date.submitted2020-06-05
dc.identifier.citationBertoncini, A., Laptenok, S. P., Genchi, L., Rajamanickam, V. P., & Liberale, C. (2020). 3D-printed high-NA catadioptric thin lens for suppression of XPM background in Stimulated Raman Scattering microscopy. Journal of Biophotonics. doi:10.1002/jbio.202000219
dc.identifier.issn1864-063X
dc.identifier.issn1864-0648
dc.identifier.doi10.1002/jbio.202000219
dc.identifier.doi10.1002/jbio.202070032
dc.identifier.urihttp://hdl.handle.net/10754/664173
dc.description.abstractStimulated Raman Scattering (SRS) is a fast chemical imaging technique with remarkable bio-science applications. Cross Phase Modulation (XPM) is a ubiquitous non-linear phenomenon that can create spurious background signals that render difficult a high-contrast imaging in SRS measurements. The XPM-induced signal is usually suppressed using high Numerical Aperture (NA) microscope objectives or condensers to collect the transmitted excitation beam. However, these high-NA optics feature short working distances, hence they are not compatible with stage-top incubators, that are necessary to perform live-cell time-lapse experiments in controlled environments. Here,we showa 3D printed high-NAcompact catadioptric lens that fits inside stage-top incubators and allows the collection of XPM-free SRS signals. The lens delivers SRS images and spectra with a quality comparable to a signal collection with a high-NA microscope objective. We also demonstrate the compatibility of the 3D printed lens with other non-linear microscopies usually associated with SRS in multimodal microscopes.
dc.description.sponsorshipWe gratefully acknowledge financial support from KingAbdullah University of Science and Technology (KAUST)Office of Sponsored Research (OSR) under Award No. OSR-2016-CRG5-3017.
dc.publisherWiley
dc.relation.urlhttps://onlinelibrary.wiley.com/doi/abs/10.1002/jbio.202000219
dc.rightsArchived with thanks to Journal of Biophotonics
dc.title3D-printed high-NA catadioptric thin lens for suppression of XPM background in Stimulated Raman Scattering microscopy
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience
dc.contributor.departmentBioscience Program
dc.contributor.departmentLabel-Free Optical Microscopy for Biology Lab
dc.identifier.journalJournal of Biophotonics
dc.eprint.versionPre-print
kaust.personBertoncini, Andrea
kaust.personLaptenok, Siarhei
kaust.personGenchi, Luca
kaust.personRajamanickam, Vijayakumar Palanisamy
kaust.personLiberale, Carlo
kaust.grant.numberOSR-2016-CRG5-3017
dc.date.accepted2020-05-09
refterms.dateFOA2020-07-14T10:02:31Z
kaust.acknowledged.supportUnitOSR
kaust.acknowledged.supportUnitSponsored Research


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