3D-printed high-NA catadioptric thin lens for suppression of XPM background in Stimulated Raman Scattering microscopy

Abstract
Stimulated Raman Scattering (SRS) is a fast chemical imaging technique with remarkable bio-science applications. Cross Phase Modulation (XPM) is a ubiquitous non-linear phenomenon that can create spurious background signals that render difficult a high-contrast imaging in SRS measurements. The XPM-induced signal is usually suppressed using high Numerical Aperture (NA) microscope objectives or condensers to collect the transmitted excitation beam. However, these high-NA optics feature short working distances, hence they are not compatible with stage-top incubators, that are necessary to perform live-cell time-lapse experiments in controlled environments. Here,we showa 3D printed high-NAcompact catadioptric lens that fits inside stage-top incubators and allows the collection of XPM-free SRS signals. The lens delivers SRS images and spectra with a quality comparable to a signal collection with a high-NA microscope objective. We also demonstrate the compatibility of the 3D printed lens with other non-linear microscopies usually associated with SRS in multimodal microscopes.

Citation
Bertoncini, A., Laptenok, S. P., Genchi, L., Rajamanickam, V. P., & Liberale, C. (2020). 3D-printed high-NA catadioptric thin lens for suppression of XPM background in Stimulated Raman Scattering microscopy. Journal of Biophotonics. doi:10.1002/jbio.202000219

Acknowledgements
We gratefully acknowledge financial support from KingAbdullah University of Science and Technology (KAUST)Office of Sponsored Research (OSR) under Award No. OSR-2016-CRG5-3017.

Publisher
Wiley

Journal
Journal of Biophotonics

DOI
10.1002/jbio.202000219
10.1002/jbio.202070032

Additional Links
https://onlinelibrary.wiley.com/doi/abs/10.1002/jbio.202000219

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