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dc.contributor.authorAman, Rashid
dc.contributor.authorAli, Zahir
dc.contributor.authorButt, Haroon
dc.contributor.authorMahas, Ahmed
dc.contributor.authorAljedaani, Fatimah R.
dc.contributor.authorKhan, Muhammad Zuhaib
dc.contributor.authorDing, Shouwei
dc.contributor.authorMahfouz, Magdy M.
dc.date.accessioned2020-07-08T14:48:13Z
dc.date.available2020-07-08T14:48:13Z
dc.date.issued2018
dc.identifier.citationAman, R., Ali, Z., Butt, H., Mahas, A., Aljedaani, F., Khan, M., Shouwei Ding, & Magdy Mahfouz. (2018). RNA virus interference via CRISPR/Cas13a system in plants. Figshare. https://doi.org/10.6084/M9.FIGSHARE.C.3968844.V1
dc.identifier.doi10.6084/m9.figshare.c.3968844.v1
dc.identifier.urihttp://hdl.handle.net/10754/664094
dc.description.abstractAbstract Background CRISPR/Cas systems confer immunity against invading nucleic acids and phages in bacteria and archaea. CRISPR/Cas13a (known previously as C2c2) is a class 2 type VI-A ribonuclease capable of targeting and cleaving single-stranded RNA (ssRNA) molecules of the phage genome. Here, we employ CRISPR/Cas13a to engineer interference with an RNA virus, Turnip Mosaic Virus (TuMV), in plants. Results CRISPR/Cas13a produces interference against green fluorescent protein (GFP)-expressing TuMV in transient assays and stable overexpression lines of Nicotiana benthamiana. CRISPR RNA (crRNAs) targeting the HC-Pro and GFP sequences exhibit better interference than those targeting other regions such as coat protein (CP) sequence. Cas13a can also process pre-crRNAs into functional crRNAs. Conclusions Our data indicate that CRISPR/Cas13a can be used for engineering interference against RNA viruses, providing a potential novel mechanism for RNA-guided immunity against RNA viruses and for other RNA manipulations in plants.
dc.publisherfigshare
dc.subjectBiophysics
dc.subjectBiochemistry
dc.subjectMicrobiology
dc.subjectGenetics
dc.subjectMolecular Biology
dc.subjectEvolutionary Biology
dc.subjectImmunology
dc.subject111714 Mental Health
dc.subject110309 Infectious Diseases
dc.subjectPlant Biology
dc.subject60506 Virology
dc.titleRNA virus interference via CRISPR/Cas13a system in plants
dc.typeDataset
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentDesert Agriculture Initiative
dc.contributor.departmentLaboratory for Genome Engineering
dc.contributor.departmentLaboratory for Genome Engineering, Division of Biological Sciences, 4700 King Abdullah University of Science and Technology, Thuwal, 23955-6900, Saudi Arabia.
dc.contributor.departmentPlant Science
dc.contributor.institutionCenter for Plant Cell Biology, Department of Microbiology and Plant Pathology, University of California, Riverside, CA, 92521, USA.
kaust.personAman, Rashid
kaust.personAli, Zahir
kaust.personButt, Haroon
kaust.personMahas, Ahmed
kaust.personAljedaani, Fatimah
kaust.personKhan, Muhammad Zuhaib
kaust.personMahfouz, Magdy M.
dc.relation.issupplementtoDOI:10.1186/s13059-017-1381-1
display.relations<b> Is Supplement To:</b><br/> <ul> <li><i>[Article]</i> <br/> Aman R, Ali Z, Butt H, Mahas A, Aljedaani F, et al. (2018) RNA virus interference via CRISPR/Cas13a system in plants. Genome Biology 19. Available: http://dx.doi.org/10.1186/s13059-017-1381-1.. DOI: <a href="https://doi.org/10.1186/s13059-017-1381-1" >10.1186/s13059-017-1381-1</a> HANDLE: <a href="http://hdl.handle.net/10754/626159">10754/626159</a></li></ul>


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