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dc.contributor.authorMoreno, Juan C
dc.contributor.authorMi, Jianing
dc.contributor.authorAgrawal, Shreya
dc.contributor.authorKössler, Stella
dc.contributor.authorTurečková, Veronika
dc.contributor.authorTarkowská, Danuše
dc.contributor.authorThiele, Wolfram
dc.contributor.authorAl-Babili, Salim
dc.contributor.authorBock, Ralph
dc.contributor.authorSchöttler, Mark Aurel
dc.date.accessioned2020-07-08T07:50:27Z
dc.date.available2020-07-08T07:50:27Z
dc.date.issued2020-08
dc.identifier.citationMoreno, J. C., Mi, J., Agrawal, S., Kössler, S., Turečková, V., Tarkowská, D., … Schöttler, M. A. (2020). Expression of a carotenogenic gene allows faster biomass production by redesigning plant architecture and improving photosynthetic efficiency in tobacco. The Plant Journal. doi:10.1111/tpj.14909
dc.identifier.issn0960-7412
dc.identifier.pmid32623777
dc.identifier.doi10.1111/tpj.14909
dc.identifier.urihttp://hdl.handle.net/10754/664081
dc.description.abstractBecause carotenoids act as accessory pigments in photosynthesis, play a key photoprotective role, and are of major nutritional importance, carotenogenesis has been a target for crop improvement. Although carotenoids are important precursors of phytohormones, previous genetic manipulations reported little if any effects on biomass production and plant development, but resulted in specific modifications in carotenoid content. Unexpectedly, the expression of the carrot lycopene b-cyclase (DcLCYB1) in Nicotiana tabacum cv. Xanthi not only resulted in increased carotenoid accumulation, but also in altered plant architecture characterized by longer internodes, faster plant growth, early flowering and increased biomass. Here, we have challenged these transformants with a range of growth conditions to determine the robustness of their phenotype and analyze the underlying mechanisms. Transgenic DcLCYB1 lines showed increased transcript levels of key genes involved in carotenoid, chlorophyll, gibberellin (GA) and abscisic acid (ABA) biosynthesis, but also in photosynthesis-related genes. Accordingly, their carotenoid, chlorophyll, ABA and GA contents were increased. Hormone application and inhibitor experiments confirmed the key role of altered GA/ABA contents in the growth phenotype. Because the longer internodes reduce shading of mature leaves, induction of leaf senescence was delayed, and mature leaves maintained a high photosynthetic capacity. This increased total plant assimilation, as reflected in higher plant yields under both fully-controlled constant and fluctuating light, and in non-controlled conditions. Furthermore, our data is a warning that engineering of isoprenoid metabolism can cause complex changes in phytohormone homeostasis and therefore plant development, which have not been sufficiently considered in previous studies.
dc.publisherWiley
dc.relation.urlhttps://onlinelibrary.wiley.com/doi/abs/10.1111/tpj.14909
dc.rightsArchived with thanks to The Plant journal : for cell and molecular biology
dc.titleExpression of a carotenogenic gene allows faster biomass production by redesigning plant architecture and improving photosynthetic efficiency in tobacco.
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentPlant Science
dc.contributor.departmentDesert Agriculture Initiative
dc.identifier.journalThe Plant journal : for cell and molecular biology
dc.rights.embargodate2021-07-06
dc.eprint.versionPost-print
dc.contributor.institutionMax Planck Institut für Molekulare Pflanzenphysiologie Am Mühlenberg 1 14476 Potsdam-Golm Germany
dc.contributor.institutionSection of Biomolecular Sciences Department of Biology University of Copenhagen Ole Maaløes Vej 5 DK-2200 Copenhagen N Denmark
dc.contributor.institutionDepartment of Biochemistry and Biology University of Potsdam Karl-Liebknecht-Straße 24-25 14476 Potsdam Germany
dc.contributor.institutionLaboratory of Growth Regulators The Czech Academy of Sciences Institute of Experimental Botany & Palacký University Šlechtitelů 27 CZ-78371 Olomouc Czech Republic
kaust.personMi, Jianing
kaust.personAl-Babili, Salim
refterms.dateFOA2020-07-08T07:50:57Z
dc.date.published-online2020-08
dc.date.published-print2020-09


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