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    Expression of a carotenogenic gene allows faster biomass production by redesigning plant architecture and improving photosynthetic efficiency in tobacco.

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    tpj.14909.pdf
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    Description:
    Accepted manuscript
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    Type
    Article
    Authors
    Moreno, Juan C cc
    Mi, Jianing cc
    Agrawal, Shreya
    Kössler, Stella
    Turečková, Veronika
    Tarkowská, Danuše
    Thiele, Wolfram
    Al-Babili, Salim cc
    Bock, Ralph
    Schöttler, Mark Aurel
    KAUST Department
    Biological and Environmental Science and Engineering (BESE) Division
    Center for Desert Agriculture
    Plant Science
    Date
    2020-08
    Online Publication Date
    2020-08
    Print Publication Date
    2020-09
    Embargo End Date
    2021-07-06
    Permanent link to this record
    http://hdl.handle.net/10754/664081
    
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    Abstract
    Because carotenoids act as accessory pigments in photosynthesis, play a key photoprotective role, and are of major nutritional importance, carotenogenesis has been a target for crop improvement. Although carotenoids are important precursors of phytohormones, previous genetic manipulations reported little if any effects on biomass production and plant development, but resulted in specific modifications in carotenoid content. Unexpectedly, the expression of the carrot lycopene b-cyclase (DcLCYB1) in Nicotiana tabacum cv. Xanthi not only resulted in increased carotenoid accumulation, but also in altered plant architecture characterized by longer internodes, faster plant growth, early flowering and increased biomass. Here, we have challenged these transformants with a range of growth conditions to determine the robustness of their phenotype and analyze the underlying mechanisms. Transgenic DcLCYB1 lines showed increased transcript levels of key genes involved in carotenoid, chlorophyll, gibberellin (GA) and abscisic acid (ABA) biosynthesis, but also in photosynthesis-related genes. Accordingly, their carotenoid, chlorophyll, ABA and GA contents were increased. Hormone application and inhibitor experiments confirmed the key role of altered GA/ABA contents in the growth phenotype. Because the longer internodes reduce shading of mature leaves, induction of leaf senescence was delayed, and mature leaves maintained a high photosynthetic capacity. This increased total plant assimilation, as reflected in higher plant yields under both fully-controlled constant and fluctuating light, and in non-controlled conditions. Furthermore, our data is a warning that engineering of isoprenoid metabolism can cause complex changes in phytohormone homeostasis and therefore plant development, which have not been sufficiently considered in previous studies.
    Citation
    Moreno, J. C., Mi, J., Agrawal, S., Kössler, S., Turečková, V., Tarkowská, D., … Schöttler, M. A. (2020). Expression of a carotenogenic gene allows faster biomass production by redesigning plant architecture and improving photosynthetic efficiency in tobacco. The Plant Journal. doi:10.1111/tpj.14909
    Publisher
    Wiley
    Journal
    The Plant Journal
    DOI
    10.1111/tpj.14909
    PubMed ID
    32623777
    Additional Links
    https://onlinelibrary.wiley.com/doi/abs/10.1111/tpj.14909
    ae974a485f413a2113503eed53cd6c53
    10.1111/tpj.14909
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division; Center for Desert Agriculture

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