Additional file 2: Sequence S1: of RNA virus interference via CRISPR/Cas13a system in plants
Aljedaani, Fatimah R.
Khan, Muhammad Zuhaib
Mahfouz, Magdy M.
KAUST DepartmentBiological and Environmental Science and Engineering (BESE) Division
Center for Desert Agriculture
Laboratory for Genome Engineering
Laboratory for Genome Engineering, Division of Biological Sciences, 4700 King Abdullah University of Science and Technology, Thuwal, 23955-6900, Saudi Arabia.
Permanent link to this recordhttp://hdl.handle.net/10754/664078
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AbstractpCas13a amino acid sequence (3xHA-pCas13a-nls). Sequence S2: pCas13a full-length plant codon optimized DNA sequence (3x-HA-pCas13a-nls) Map S1: pCas13a sequence in pK2GW7-pCas13a (pK2GW7-3xHA-pCas13a-nls). Sequence S3: TuMV-GFP full-length sequence with target sequences in different colors. Map S2: Map of TuMV-GFP. Sequence S4: Cas13a-repeat-cRNA-TuMV-GFP-GFP-target 1 sequence (A), map (B) and its complex with targeting region in TuMV GFP genomic region (C). Sequence S5: Cas13a-repeat-cRNA-TuMV-GFP-GFP-T2 sequence (A), map (B), and its complex with targeting area in TuMV GFP genomic region (C). Sequence S6: Cas13a-repeat-cRNA-TuMV-GFP-HC-Pro-T1 sequence (A), map (B), and its complex with HC-pro targeting region in TuMV (C). Sequence S7: Cas13a-repeat-cRNA-TuMV-GFP-Cp-Pro-T1 sequence (A), map (B), and its complex with Capsid protein targeting region in TuMV. (DOCX 414 kb)
CitationAman, R., Ali, Z., Butt, H., Mahas, A., Aljedaani, F., Khan, M., Shouwei Ding, & Magdy Mahfouz. (2018). Additional file 2: Sequence S1: of RNA virus interference via CRISPR/Cas13a system in plants. Figshare. https://doi.org/10.6084/M9.FIGSHARE.5759157.V1
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