Simultaneous Measurements of Dinitrogen Fixation and Denitrification Associated With Coral Reef Substrates: Advantages and Limitations of a Combined Acetylene Assay
AuthorsEl-Khaled, Yusuf C.
Kharbatia, Najeh M.
Voolstra, Christian R.
KAUST DepartmentMarine Science Program
Biological and Environmental Sciences and Engineering (BESE) Division
Red Sea Research Center (RSRC)
Permanent link to this recordhttp://hdl.handle.net/10754/664026
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AbstractNitrogen (N) cycling in coral reefs is of key importance for these oligotrophic ecosystems, but knowledge about its pathways is limited. While dinitrogen (N2) fixation is comparably well studied, the counteracting denitrification pathway is under-investigated, mainly because of expensive and relatively complex experimental techniques currently available. Here, we combined two established acetylene-based assays to one single setup to determine N2-fixation and denitrification performed by microbes associated with coral reef substrates/organisms simultaneously. Accumulating target gases (ethylene for N2-fixation, nitrous oxide for denitrification) were measured in gaseous headspace samples via gas chromatography. We measured N2-fixation and denitrification rates of two Red Sea coral reef substrates (filamentous turf algae, coral rubble), and demonstrated, for the first time, the co-occurrence of both N-cycling processes in both substrates. N2-fixation rates were up to eight times higher during the light compared to the dark, whereas denitrification rates during dark incubations were stimulated for turf algae and suppressed for coral rubble compared to light incubations. Our results highlight the importance of both substrates in fixing N, but their role in relieving N is potentially divergent. Absolute N2-fixation rates of the present study correspond with rates reported previously, even though likely underestimated due to an initial lag phase. Denitrification is also presumably underestimated due to incomplete nitrous oxide inhibition and/or substrate limitation. Besides these inherent limitations, we show that a relative comparison of N2-fixation and denitrification activity between functional groups is possible. Thus, our approach facilitates cost-efficient sample processing in studies interested in comparing relative rates of N2-fixation and denitrification.
CitationEl-Khaled, Y. C., Roth, F., Rädecker, N., Kharbatia, N., Jones, B. H., Voolstra, C. R., & Wild, C. (2020). Simultaneous Measurements of Dinitrogen Fixation and Denitrification Associated With Coral Reef Substrates: Advantages and Limitations of a Combined Acetylene Assay. Frontiers in Marine Science, 7. doi:10.3389/fmars.2020.00411
SponsorsWe are grateful to Arjen Tilstra, Rodrigo Villalobos, João Cúrdia, and Denis B. Karcher for support during fieldwork. Many thanks also to Söphiä Tobler, Rainer Willhaus, and Jan Krause for their help in developing the figures. Further, we would like to thank the editor as well as the two reviewers for their constructive comments on the manuscript. Funding. This work was funded by the German Research Association (DFG Project: Wi 2677/9-1) to CW and KAUST baseline funding to BJ and CV.
PublisherFrontiers Media SA
JournalFrontiers in Marine Science