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dc.contributor.authorRamos Mandujano, Gerardo
dc.contributor.authorSalunke, Rahul
dc.contributor.authorMfarrej, Sara
dc.contributor.authorRachmadi, Andri Taruna
dc.contributor.authorHala, Sharif
dc.contributor.authorXu, Jinna
dc.contributor.authorAlofi, Fadwa S
dc.contributor.authorKhogeer, Asim
dc.contributor.authorHashem, Anwar M
dc.contributor.authorAlmontashiri, Naif AM
dc.contributor.authorAlsomali, Afrah
dc.contributor.authorHamdan, Samir
dc.contributor.authorHong, Pei-Ying
dc.contributor.authorPain, Arnab
dc.contributor.authorLi, Mo
dc.identifier.citationRamos-Mandujano, G., Salunke, R., Mfarrej, S., Rachmadi, A., Hala, S., Xu, J., … Li, M. (2020). A Robust, Safe and Scalable Magnetic Nanoparticle Workflow for RNA Extraction of Pathogens from Clinical and Environmental Samples. doi:10.1101/2020.06.28.20141945
dc.description.abstractDiagnosis and surveillance of emerging pathogens such as SARS-CoV-2 depend on nucleic acid isolation from clinical and environmental samples. Under normal circumstances, samples would be processed using commercial proprietary reagents in Biosafety 2 (BSL-2) or higher facilities. A pandemic at the scale of COVID-19 has caused a global shortage of proprietary reagents and BSL-2 laboratories to safely perform testing. Therefore, alternative solutions are urgently needed to address these challenges. We developed an open-source method called Magnetic- nanoparticle-Aided Viral RNA Isolation of Contagious Samples (MAVRICS) that is built upon reagents that are either readily available or can be synthesized in any molecular biology laboratory with basic equipment. Unlike conventional methods, MAVRICS works directly in samples inactivated in acid guanidinium thiocyanate-phenol-chloroform (e.g., TRIzol), thus allowing infectious samples to be handled safely without biocontainment facilities. Using 36 COVID-19 patient samples, 2 wastewater samples and 1 human pathogens control sample, we showed that MAVRICS rivals commercial kits in validated diagnostic tests of SARS-CoV-2, influenza viruses, and respiratory syncytial virus. MAVRICS is scalable and thus could become an enabling technology for widespread community testing and wastewater monitoring in the current and future pandemics.
dc.publisherCold Spring Harbor Laboratory
dc.rightsArchived with thanks to Cold Spring Harbor Laboratory
dc.titleA Robust, Safe and Scalable Magnetic Nanoparticle Workflow for RNA Extraction of Pathogens from Clinical and Environmental Samples
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentEnvironmental Microbial Safety and Biotechnology Lab
dc.contributor.departmentEnvironmental Science and Engineering Program
dc.contributor.departmentLaboratory of DNA Replication and Recombination
dc.contributor.departmentPathogen Genomics Laboratory
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)
dc.contributor.institutionInfectious Diseases Department, King Fahad Hospital, Madinah, Saudi Arabia.
dc.contributor.institutionKing Abdullah International Medical Research Centre, Jeddah, Makkah, Ministry of National Guard Health Affairs, Jeddah, Makkah, Saudi Arabia.
dc.contributor.institutionCollege of Applied Medical Sciences, Taibah University, Madinah, Saudi Arabia.
dc.contributor.institutionKing Abdullah Medical Complex (KAMC), Jeddah, Saudi Arabia.
kaust.personRamos Mandujano, Gerardo
kaust.personSalunke, Rahul
kaust.personMfarrej, Sara
kaust.personRachmadi, Andri Taruna
kaust.personHala, Sharif
kaust.personXu, Jinna
kaust.personHamdan, Samir
kaust.personHong, Pei-Ying
kaust.personPain, Arnab
kaust.personLi, Mo

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