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dc.contributor.authorParrotta, Elvira
dc.contributor.authorDe Angelis, Maria Teresa
dc.contributor.authorScalise, Stefania
dc.contributor.authorCandeloro, Patrizio
dc.contributor.authorSantamaria, Gianluca
dc.contributor.authorPaonessa, Mariagrazia
dc.contributor.authorColuccio, Maria Laura
dc.contributor.authorPerozziello, Gerardo
dc.contributor.authorDe Vitis, Stefania
dc.contributor.authorSgura, Antonella
dc.contributor.authorColuzzi, Elisa
dc.contributor.authorMollace, Vincenzo
dc.contributor.authorDi Fabrizio, Enzo M.
dc.contributor.authorCuda, Giovanni
dc.date.accessioned2020-06-30T13:32:41Z
dc.date.available2020-06-30T13:32:41Z
dc.date.issued2017
dc.identifier.citationParrotta, E., Angelis, M. D., Scalise, S., Candeloro, P., Santamaria, G., Mariagrazia Paonessa, Coluccio, M., Perozziello, G., Vitis, S. D., Sgura, A., Coluzzi, E., Mollace, V., Fabrizio, E. D., & Cuda, G. (2017). Additional file 1: Figure S1. of Two sides of the same coin? Unraveling subtle differences between human embryonic and induced pluripotent stem cells by Raman spectroscopy. figshare. https://doi.org/10.6084/M9.FIGSHARE.5643631.V1
dc.identifier.doi10.6084/m9.figshare.5643631.v1
dc.identifier.urihttp://hdl.handle.net/10754/663945
dc.description.abstractShowing generation and characterization of hiPSCs from skin fibroblasts. (A) Representative images of generated iPSCs showing ESC-like morphology and AP activity. (B) RT-PCR analysis showing loss of Sendai viral transgenes in hiPSCs (lane 1), their presence in infected parental fibroblasts (ipF, lane 2), and their absence in noninfected parental fibroblasts (pF, lane 3). (C) qRT-PCR analysis confirming upregulation of the endogenous pluripotency genes OCT4, SOX2, c-MYC, REX1, and NANOG in generated hiPSCs and hESCs, which are used as a control cell line. All expression values normalized to GAPDH and relative to the parental fibroblasts. Data represent mean ± SD. (D) Immunostaining of hESCs and iPSCs for pluripotency markers Oct4 (red) and Nanog (green), with costaining with DAPI (blue). Scale bar = 50 μm. (E) Genome-wide gene expression profiling by PluriTest of undifferentiated hESCs and iPSCs reveals that the lines have a high pluripotency score. (F) Immunostaining of whole EBs (day 25) for Nestin (ectoderm), Brachyury (mesoderm), and Sox17 (endoderm) and costaining with DAPI (blue). Scale bar = 50 μm. (G) qRT-PCR analysis showing potential of generated hiPSCs to differentiate into cells of all three germ layers (SOX7, endodermal marker; HAND1, ACTA2, and MYL2, mesodermal markers; NESTIN and BMP4, ectodermal markers) and downregulation of pluripotency-associated genes (OCT4 and NANOG). All expression values normalized to GAPDH and relative to the respective hiPSC clones (TIF 5709 kb)
dc.publisherfigshare
dc.subjectCell Biology
dc.subjectGenetics
dc.subjectMolecular Biology
dc.subjectNeuroscience
dc.subjectDevelopmental Biology
dc.subject111714 Mental Health
dc.titleAdditional file 1: Figure S1. of Two sides of the same coin? Unraveling subtle differences between human embryonic and induced pluripotent stem cells by Raman spectroscopy
dc.typeData File
dc.contributor.departmentPhysical Science and Engineering (PSE) Division
dc.contributor.departmentMaterial Science and Engineering Program
dc.contributor.institutionResearch Center for Advanced Biochemistry and Molecular Biology, Stem Cell Laboratory, Department of Experimental and Clinical Medicine, University “Magna Graecia” of Catanzaro, 88100 Loc., Germaneto, Catanzaro, Italy.
dc.contributor.institutionBioNEM Lab., Department of Experimental and Clinical Medicine, University “Magna Graecia” of Catanzaro, 88100 Loc., Germaneto, Catanzaro, Italy.
dc.contributor.institutionDepartment of Science, University of Rome “Roma tre”, viale G. Marconi 446, 00146 Rome, Italy.
dc.contributor.institutionDepartment of Health Sciences, University “Magna Graecia” of Catanzaro, 88100 Loc., Germaneto, Catanzaro, Italy.
kaust.personDi Fabrizio, Enzo M.
dc.relation.issupplementtoDOI:10.1186/s13287-017-0720-1
display.relations<b> Is Supplement To:</b><br/> <ul> <li><i>[Article]</i> <br/> Parrotta E, De Angelis MT, Scalise S, Candeloro P, Santamaria G, et al. (2017) Two sides of the same coin? Unraveling subtle differences between human embryonic and induced pluripotent stem cells by Raman spectroscopy. Stem Cell Research & Therapy 8. Available: http://dx.doi.org/10.1186/s13287-017-0720-1.. DOI: <a href="https://doi.org/10.1186/s13287-017-0720-1" >10.1186/s13287-017-0720-1</a> HANDLE: <a href="http://hdl.handle.net/10754/626288">10754/626288</a></li></ul>


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