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dc.contributor.authorDiBattista, Joseph D.
dc.contributor.authorCoker, Darren James
dc.contributor.authorSinclair-Taylor, Tane
dc.contributor.authorStat, Michael
dc.contributor.authorBerumen, Michael L.
dc.contributor.authorBunce, Michael
dc.date.accessioned2020-06-25T13:30:37Z
dc.date.available2020-06-25T13:30:37Z
dc.date.issued2018
dc.identifier.citationDiBattista, J. D., Coker, D. J., Sinclair-Taylor, T. H., Stat, M., Berumen, M. L., & Bunce, M. (2018). Data from: Assessing the utility of eDNA as a tool to survey reef-fish communities in the Red Sea (Version 1) [Data set]. Dryad. https://doi.org/10.5061/DRYAD.1PM20
dc.identifier.doi10.5061/dryad.1pm20
dc.identifier.urihttp://hdl.handle.net/10754/663861
dc.description.abstractRelatively small volumes of water may contain sufficient environmental DNA (eDNA) to detect target aquatic organisms via genetic sequencing. We therefore assessed the utility of eDNA to document the diversity of coral reef fishes in the central Red Sea. DNA from seawater samples was extracted, amplified using fish-specific 16S mitochondrial DNA primers, and sequenced using a metabarcoding workflow. DNA sequences were assigned to taxa using available genetic repositories or custom genetic databases generated from reference fishes. Our approach revealed a diversity of conspicuous, cryptobenthic, and commercially relevant reef fish at the genus level, with select genera in the family Labridae over-represented. Our approach, however, failed to capture a significant fraction of the fish fauna known to inhabit the Red Sea, which we attribute to limited spatial sampling, amplification stochasticity, and an apparent lack of sequencing depth. Given an increase in fish species descriptions, completeness of taxonomic checklists, and improvement in species-level assignment with custom genetic databases as shown here, we suggest that the Red Sea region may be ideal for further testing of the eDNA approach.
dc.publisherDryad
dc.subjectMetabarcoding
dc.subjectbiodiversity
dc.subjectEnvironmental DNA
dc.subjectNext-generation sequencing
dc.subjectCoral reef
dc.titleData from: Assessing the utility of eDNA as a tool to survey reef-fish communities in the Red Sea
dc.typeDataset
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentMarine Science Program
dc.contributor.departmentRed Sea Research Center (RSRC)
dc.contributor.departmentReef Ecology Lab
dc.contributor.institutionTrace and Environmental DNA Laboratory, Department of Environment and Agriculture, Curtin University, Perth, Australia
kaust.personCoker, Darren James
kaust.personSinclair-Taylor, Tane
kaust.personBerumen, Michael L.
dc.relation.issupplementtoDOI:10.1007/s00338-017-1618-1
display.relations<b> Is Supplement To:</b><br/> <ul> <li><i>[Article]</i> <br/> DiBattista JD, Coker DJ, Sinclair-Taylor TH, Stat M, Berumen ML, et al. (2017) Assessing the utility of eDNA as a tool to survey reef-fish communities in the Red Sea. Coral Reefs. Available: http://dx.doi.org/10.1007/s00338-017-1618-1.. DOI: <a href="https://doi.org/10.1007/s00338-017-1618-1" >10.1007/s00338-017-1618-1</a> HANDLE: <a href="http://hdl.handle.net/10754/625746">10754/625746</a></li></ul>


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