Attempting to synthesize lasso peptides using high pressure

Lasso peptides are unique in that the tail of the lasso peptide threads through its macrolactam ring. The unusual structure and biological activity of lasso peptides have generated increased interest from the scientific community in recent years. Because of this, many new types of lasso peptides have been discovered. These peptides can be synthesized by microorganisms efficiently, and yet, their chemical assembly is challenging. Herein, we investigated the possibility of high pressure inducing the cyclization of linear precursors of lasso peptides. Unlike other molecules like rotaxanes which mechanically interlock at high pressure, the threaded lasso peptides did not form, even at pressures the high pressure up to 14000 kbar.

Waliczek, M., Wierzbicka, M., Arkuszewski, M., Kijewska, M., Jaremko, Ł., Rajagopal, P., … Stefanowicz, P. (2020). Attempting to synthesize lasso peptides using high pressure. PLOS ONE, 15(6), e0234901. doi:10.1371/journal.pone.0234901

This work was supported by Grant No.UMO-2015/19/B/ST5/00659 from the National Science Centre, Poland The co-authors: Łukasz Jaremko, Mariusz Jaremko, Priyadharshni Rajagopal and Kacper Szczepski are grateful to King Abdullah University of Science and Technology (KAUST) for financial support. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Public Library of Science (PLoS)



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