Standardized short-term acute heat stress assays resolve historical differences in coral thermotolerance across microhabitat reef sites
AuthorsVoolstra, Christian R.
Barshis, Daniel J.
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Marine Science Program
Red Sea Research Center (RSRC)
Red Sea Research Center Division of Biological BESE King Abdullah University of Science and Technology (KAUST) Thuwal Saudi Arabia
Reef Genomics Lab
Online Publication Date2020-06-21
Print Publication Date2020-08
Permanent link to this recordhttp://hdl.handle.net/10754/663781
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AbstractCoral bleaching is one of the main drivers of reef degradation. Most corals bleach and suffer mortality at just 1–2°C above their maximum monthly mean temperatures, but some species and genotypes resist or recover better than others. Here, we conducted a series of 18-hr short-term acute heat stress assays side-by-side with a 21-day long-term heat stress experiment to assess the ability of both approaches to resolve coral thermotolerance differences reflective of in situ reef temperature thresholds. Using a suite of physiological parameters (photosynthetic efficiency, coral whitening, chlorophyll a , host protein, algal symbiont counts, and algal type association), we assessed bleaching susceptibility of Stylophora pistillata colonies from the windward/exposed and leeward/protected sites of a nearshore coral reef in the central Red Sea, which had previously shown differential mortality during a natural bleaching event. Photosynthetic efficiency was most indicative of the expected higher thermal tolerance in corals from the protected reef site, denoted by an increased retention of dark-adapted maximum quantum yields at higher temperatures. These differences were resolved using both experimental setups, as corroborated by a positive linear relationship, not observed for the other parameters. Notably, short-term acute heat stress assays resolved per-colony (genotype) differences that may have been masked by acclimation effects in the long-term experiment. Using our newly developed portable experimental system termed the Coral Bleaching Automated Stress System (CBASS), we thus highlight the potential of mobile, standardized short-term acute heat stress assays to resolve fine-scale differences in coral thermotolerance. Accordingly, such a system may be suitable for large-scale determination and complement existing approaches to identify resilient genotypes/reefs for downstream experimental examination and prioritization of reef sites for conservation/restoration. Development of such a framework is consistent with the recommendations of the National Academy of Sciences and the Reef Restoration and Adaptation Program committees for new intervention and restoration strategies.
CitationVoolstra, C. R., Buitrago-López, C., Perna, G., Cárdenas, A., Hume, B. C. C., Rädecker, N., & Barshis, D. J. (2020). Standardized short-term acute heat stress assays resolve historical differences in coral thermotolerance across microhabitat reef sites. Global Change Biology. doi:10.1111/gcb.15148
SponsorsPart of this study was conducted as the master’s thesis of G.P. This project was supported by research funding from King Abdullah University of Science and Technology to C.R.V. and a Binational Science Foundation award (2016403) to D.J.B. We would like to thank Katherine Rowe for assistance with field and lab work. We would also like to thank the Bioscience Core Lab (BCL) for sequenc-ing and CMOR for assistance with aquaria experiments and boating operations, in particular Zenon Batang and Nabeel Alikuhni.
JournalGlobal Change Biology
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