Extraction, Characterization, and Anticoagulant Activity of a Sulfated Polysaccharide from Bursatella leachii Viscera
Type
ArticleAuthors
Dhahri, Manel
Sioud, Salim
Dridi, Rihab
Hassine, Mohsen
Boughattas, Naceur A.
Almulhim, Fatimah F.

Al-Talla, Zeyad
Jaremko, Mariusz

Emwas, Abdul-Hamid M.
KAUST Department
Ali I. Al-Naimi Petroleum Engineering Research Center (ANPERC)Biological and Environmental Sciences and Engineering (BESE) Division
Bioscience
Bioscience Program
NMR
Organics
Physical Science and Engineering (PSE) Division
KAUST Grant Number
BAS/1/1085-01-01Date
2020-06-10Online Publication Date
2020-06-10Print Publication Date
2020-06-23Permanent link to this record
http://hdl.handle.net/10754/663499
Metadata
Show full item recordAbstract
Bioactive compounds for drug discovery are increasingly extracted and purified from natural sources including marine organisms. Heparin is a therapeutic agent that has been used for several decades as an anticoagulant. However, heparin is known to cause many undesirable complications such as thrombocytopenia and risk of hemorrhage. Hence, there is a need to find alternatives to current widely used anticoagulant drugs. Here, we extract a sulfated polysaccharide from sea hare, that is, Bursatella leachii viscera, by enzymatic digestion. Several analytical approaches including elemental analysis, Fourier-transform infrared spectroscopy, nuclear magnetic resonance, and high-performance liquid chromatography–mass spectrometry analysis show that B. leachii polysaccharides have chemical structures similar to glycosaminoglycans. We explore the anticoagulant activity of the B. leachii extract using the activated partial thromboplastin time and the thrombin time. Our results demonstrate that the extracted sulfated polysaccharide has heparin-like anticoagulant activity, thus showing great promise as an alternative anticoagulant therapy.Citation
Dhahri, M., Sioud, S., Dridi, R., Hassine, M., Boughattas, N. A., Almulhim, F., … Emwas, A.-H. M. (2020). Extraction, Characterization, and Anticoagulant Activity of a Sulfated Polysaccharide from Bursatella leachii Viscera. ACS Omega. doi:10.1021/acsomega.0c01724Sponsors
This work was supported by Taibah university and King Abdullah University of Science and Technology (under fund number BAS/1/1085-01-01) to M.J.Publisher
American Chemical Society (ACS)Journal
ACS OmegaAdditional Links
https://pubs.acs.org/doi/10.1021/acsomega.0c01724ae974a485f413a2113503eed53cd6c53
10.1021/acsomega.0c01724