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dc.contributor.authorButt, Haroon
dc.contributor.authorRao, Gundra Sivakrishna
dc.contributor.authorSedeek, Khalid Elwy Mohamed
dc.contributor.authorAman, Rashid
dc.contributor.authorKamel, Radwa
dc.contributor.authorMahfouz, Magdy M.
dc.date.accessioned2020-05-18T12:24:30Z
dc.date.available2020-05-18T12:24:30Z
dc.date.issued2020-06-11
dc.identifier.citationButt, H., Rao, G. S., Sedeek, K., Aman, R., Kamel, R., & Mahfouz, M. (2020). Engineering herbicide resistance via prime editing in rice. Plant Biotechnology Journal. doi:10.1111/pbi.13399
dc.identifier.issn1467-7644
dc.identifier.issn1467-7652
dc.identifier.pmid32415890
dc.identifier.doi10.1111/pbi.13399
dc.identifier.urihttp://hdl.handle.net/10754/662865
dc.description.abstractAlthough CRISPR-Cas9 has revolutionized our ability to generate site-specific double-strand breaks, precise editing of the genome remains challenging in most eukaryotes, including plants (Shan et al., 2013). In plants homology-directed repair is inefficient, limiting our ability to make precise edits of the DNA sequence (Ali et al., 2020; Butt et al., 2017). Moreover, cytosine and adenine base editors have serious drawbacks including lower efficiency, unclean edited sequence, and the possibility of off-target mutations at other loci (Rees and Liu, 2018). Chimeric single guide RNAs (sgRNAs) can provide editing information, in RNA form, but this modality suffers from several limitations including lower efficiency, less versatility, and the need for long homology arms (Butt et al., 2017).
dc.description.sponsorshipWe would like to thank members of the genome engineering and synthetic biology laboratory at KAUST for their critical discussion and technical help in this work.
dc.publisherWiley
dc.relation.urlhttps://onlinelibrary.wiley.com/doi/abs/10.1111/pbi.13399
dc.relation.urlhttps://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/pbi.13399
dc.rightsArchived with thanks to Plant Biotechnology Journal
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleEngineering herbicide resistance via prime editing in rice
dc.typeArticle
dc.contributor.departmentBiological and Environmental Science and Engineering (BESE) Division
dc.contributor.departmentBioscience
dc.contributor.departmentBioscience Program
dc.contributor.departmentCenter for Desert Agriculture
dc.contributor.departmentLaboratory for Genome Engineering
dc.contributor.departmentLaboratory for Genome Engineering and Synthetic Biology King Abdullah University of Science and Technology (KAUST) Thuwal Saudi Arabia
dc.contributor.departmentPlant Science
dc.identifier.journalPlant Biotechnology Journal
dc.eprint.versionPost-print
kaust.personButt, Haroon
kaust.personRao, Gundra Sivakrishna
kaust.personSedeek, Khalid Elwy Mohamed
kaust.personAman, Rashid
kaust.personKamel, Radwa
kaust.personMahfouz, Magdy M.
refterms.dateFOA2020-05-18T12:25:16Z
kaust.acknowledged.supportUnitgenome engineering and synthetic biology laboratory
dc.date.published-online2020-06-11
dc.date.published-print2020-12


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