Type
ArticleAuthors
Butt, Haroon
Rao, Gundra Sivakrishna
Sedeek, Khalid Elwy Mohamed

Aman, Rashid

Kamel, Radwa
Mahfouz, Magdy M.

KAUST Department
Biological and Environmental Science and Engineering (BESE) DivisionBioscience
Bioscience Program
Center for Desert Agriculture
Laboratory for Genome Engineering
Laboratory for Genome Engineering and Synthetic Biology King Abdullah University of Science and Technology (KAUST) Thuwal Saudi Arabia
Plant Science
Date
2020-06-11Online Publication Date
2020-06-11Print Publication Date
2020-12Permanent link to this record
http://hdl.handle.net/10754/662865
Metadata
Show full item recordAbstract
Although CRISPR-Cas9 has revolutionized our ability to generate site-specific double-strand breaks, precise editing of the genome remains challenging in most eukaryotes, including plants (Shan et al., 2013). In plants homology-directed repair is inefficient, limiting our ability to make precise edits of the DNA sequence (Ali et al., 2020; Butt et al., 2017). Moreover, cytosine and adenine base editors have serious drawbacks including lower efficiency, unclean edited sequence, and the possibility of off-target mutations at other loci (Rees and Liu, 2018). Chimeric single guide RNAs (sgRNAs) can provide editing information, in RNA form, but this modality suffers from several limitations including lower efficiency, less versatility, and the need for long homology arms (Butt et al., 2017).Citation
Butt, H., Rao, G. S., Sedeek, K., Aman, R., Kamel, R., & Mahfouz, M. (2020). Engineering herbicide resistance via prime editing in rice. Plant Biotechnology Journal. doi:10.1111/pbi.13399Sponsors
We would like to thank members of the genome engineering and synthetic biology laboratory at KAUST for their critical discussion and technical help in this work.Publisher
WileyJournal
Plant Biotechnology JournalPubMed ID
32415890Additional Links
https://onlinelibrary.wiley.com/doi/abs/10.1111/pbi.13399https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/pbi.13399
ae974a485f413a2113503eed53cd6c53
10.1111/pbi.13399
Scopus Count
Except where otherwise noted, this item's license is described as Archived with thanks to Plant Biotechnology Journal
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