Rao, Gundra Sivakrishna
Sedeek, Khalid Elwy Mohamed
Mahfouz, Magdy M.
KAUST DepartmentBiological and Environmental Science and Engineering (BESE) Division
Center for Desert Agriculture
Laboratory for Genome Engineering
Laboratory for Genome Engineering and Synthetic Biology King Abdullah University of Science and Technology (KAUST) Thuwal Saudi Arabia
Online Publication Date2020-06-11
Print Publication Date2020-12
Permanent link to this recordhttp://hdl.handle.net/10754/662865
MetadataShow full item record
AbstractAlthough CRISPR-Cas9 has revolutionized our ability to generate site-specific double-strand breaks, precise editing of the genome remains challenging in most eukaryotes, including plants (Shan et al., 2013). In plants homology-directed repair is inefficient, limiting our ability to make precise edits of the DNA sequence (Ali et al., 2020; Butt et al., 2017). Moreover, cytosine and adenine base editors have serious drawbacks including lower efficiency, unclean edited sequence, and the possibility of off-target mutations at other loci (Rees and Liu, 2018). Chimeric single guide RNAs (sgRNAs) can provide editing information, in RNA form, but this modality suffers from several limitations including lower efficiency, less versatility, and the need for long homology arms (Butt et al., 2017).
CitationButt, H., Rao, G. S., Sedeek, K., Aman, R., Kamel, R., & Mahfouz, M. (2020). Engineering herbicide resistance via prime editing in rice. Plant Biotechnology Journal. doi:10.1111/pbi.13399
SponsorsWe would like to thank members of the genome engineering and synthetic biology laboratory at KAUST for their critical discussion and technical help in this work.
JournalPlant Biotechnology Journal
Except where otherwise noted, this item's license is described as Archived with thanks to Plant Biotechnology Journal
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