Spatial confinement and temporal dynamics of selectin ligands enable stable hematopoietic stem cell rolling
AuthorsAl Alwan, Bader
Aldehaiman, Mansour M.
Al-Amoodi, Asma S.
Serag, Maged F.
Aleisa, Fajr A
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Single-Molecule Spectroscopy and Microscopy Research Group
KAUST Grant NumberCRG R2 13 MERZ KAUST 1
Permanent link to this recordhttp://hdl.handle.net/10754/662831
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AbstractAbstractHematopoietic stem/progenitor cell (HSPC) homing is initiated by tethering and rolling of the cells on endothelium through selectin-ligand interactions. Although multiple factors that affect the rolling behaviour of the cells have been identified, molecular mechanisms that enable slow and stable cell rolling remain elusive. Here, using a microfluidics-based single-molecule live cell fluorescence imaging, we reveal that unique spatiotemporal dynamics of selectin ligands on the membrane tethers and slings, which are distinct from that on the cell body, play an essential role in the rolling of the cell. Our results suggest that the spatial confinement of the selectin ligands to the tethers and slings together with the rapid scanning of a large area by the selectin ligands increases the efficiency of selectin-ligand interactions during cell rolling, resulting in slow and stable rolling of the cell on the selectins. Our findings provide novel insights and contribute significantly to the molecular-level understanding of the initial and essential step of the homing.
CitationAlwan, B. A., AbuZineh, K., Nozue, S., Rakhmatulina, A., Aldehaiman, M., Al-Amoodi, A. S., … Habuchi, S. (2020). Spatial confinement and temporal dynamics of selectin ligands enable stable hematopoietic stem cell rolling. doi:10.1101/2020.04.29.069617
SponsorsWe thank Ms. Ohoud M. Alharbi for the SEM images of KG1a cells. The research reported in this publication was supported by funding from the King Abdullah University of Science and Technology (KAUST) and the KAUST Office of Sponsored Research under Award No. CRG R2 13 MERZ KAUST 1. We would like to thank Ms. Samar A. Rustum and Ms. Umm Habiba for their support in the management of the lab.
PublisherCold Spring Harbor Laboratory