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dc.contributor.authorKoklu, Anil
dc.contributor.authorGiuliani, Jason
dc.contributor.authorMonton, Carlos
dc.contributor.authorBeskok, Ali
dc.date.accessioned2020-05-12T12:11:05Z
dc.date.available2020-05-12T12:11:05Z
dc.date.issued2020-05-04
dc.identifier.citationKoklu, A., Giuliani, J., Monton, C., & Beskok, A. (2020). Rapid and Sensitive Detection of Nanomolecules by AC Electrothermal Flow Facilitated Impedance Immunosensor. Analytical Chemistry. doi:10.1021/acs.analchem.0c00890
dc.identifier.issn0003-2700
dc.identifier.issn1520-6882
dc.identifier.doi10.1021/acs.analchem.0c00890
dc.identifier.urihttp://hdl.handle.net/10754/662812
dc.description.abstractConventional immunosensors typically rely on passive diffusion dominated transport of analytes for binding reaction and hence, it is limited by low sensitivity and long detection times. We report a simple and efficient impedance sensing method that can be utilized to overcome both sensitivity and diffusion limitations of immunosensors. This method incorporates the structural advantage of nanorod-covered interdigitated electrodes and the microstirring effect of AC electrothermal flow (ACET) with impedance spectroscopy. ACET flow induced by a biased AC electric field can rapidly convect the analyte onto nanorod structured electrodes within a few seconds and enriches the number of binding molecules because of excessive effective surface area. We performed numerical simulations to investigate the effect of ACET flow on the biosensor performance. The results indicated that AC bias to the side electrodes could induce fast convective flow, which facilitates the transport of the target molecules to the binding region located in the middle as a floating electrode. The temperature rise due to the Joule heating effect was measured using a thermoreflectance imaging method to find the optimum device operation conditions. The change of impedance caused by the receptors–target molecules binding at the sample/electrode interface was experimentally measured and quantified in real-time using the impedance spectroscopy technique. We observed that the impedance sensing method exhibited extremely fast response compared with those under no bias conditions. The measured impedance change can reach saturation in a minute. Compared to the conventional incubation method, the ACET flow enhanced method is faster in its reaction time, and the detection limit can be reduced to 1 ng/ml. In this work, we demonstrate that this sensor technology is promising and reliable for rapid, sensitive, and real-time monitoring biomolecules in biologically relevant media such as blood, urine, and saliva.
dc.description.sponsorshipThe authors are grateful to Mr. Assaad El Helou and Dr. Peter E. Raad for their assistance to integrate thermoreflectance method to fluidic systems. We are also thankful to SMU Hunt Institute for their support.
dc.publisherAmerican Chemical Society (ACS)
dc.relation.urlhttps://pubs.acs.org/doi/abs/10.1021/acs.analchem.0c00890
dc.rightsThis document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/doi/abs/10.1021/acs.analchem.0c00890.
dc.titleRapid and Sensitive Detection of Nanomolecules by AC Electrothermal Flow Facilitated Impedance Immunosensor
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.identifier.journalAnalytical Chemistry
dc.rights.embargodate2021-05-04
dc.eprint.versionPost-print
dc.contributor.institutionDepartment of Physics and Astronomy, the University of Texas at San Antonio, TX 78249, USA.
dc.contributor.institutionGeneral Atomics, P.O. Box 85608, San Diego, USA 92186.
dc.contributor.institutionDepartment of Mechanical Engineering, Southern Methodist University, Dallas, TX 75205, USA.
kaust.personKoklu, Anil
refterms.dateFOA2020-05-12T12:11:51Z
dc.date.published-online2020-05-04
dc.date.published-print2020-06-02


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