Transcriptional Patterns of Biogeochemically Relevant Marker Genes by Temperate Marine Bacteria.
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Marine Science Program
Microbial oceanography Research Group
Red Sea Research Center (RSRC)
Permanent link to this recordhttp://hdl.handle.net/10754/662502
MetadataShow full item record
AbstractEnvironmental microbial gene expression patterns remain largely unexplored, particularly at interannual time scales. We analyzed the variability in the expression of marker genes involved in ecologically relevant biogeochemical processes at a temperate Atlantic site over two consecutive years. Most of nifH transcripts, involved in nitrogen (N) fixation, were affiliated with the symbiotic cyanobacterium Candidatus Atelocyanobacterium thalassa, suggesting a key role as N providers in this system. The expression of nifH and amoA (i.e., marker for ammonia oxidation) showed consistent maxima in summer and autumn, respectively, suggesting a temporal succession of these important N cycling processes. The patterns of expression of genes related to the oxidation of carbon monoxide (coxL) and reduced sulfur (soxB) were different from that of amoA, indicating alternate timings for these energy conservation strategies. We detected expression of alkaline phosphatases, induced under phosphorus limitation, in agreement with the reported co-limitation by this nutrient at the study site. In contrast, low-affinity phosphate membrane transporters (pit) typically expressed under phosphorus luxury conditions, were mainly detected in post-bloom conditions. Rhodobacteraceae dominated the expression of soxB, coxL and ureases, while Pelagibacteraceae dominated the expression of proteorhodopsins. Bacteroidetes and Gammaproteobacteria were major contributors to the uptake of inorganic nutrients (pit and amt transporters). Yet, in autumn, Thauma- and Euryarchaeota unexpectedly contributed importantly to the uptake of ammonia and phosphate, respectively. We provide new hints on the active players and potential dynamics of ecologically relevant functions in situ, highlighting the potential of metatranscriptomics to provide significant input to future omics-driven marine ecosystem assessment.
CitationAlonso-Sáez, L., Morán, X. A. G., & González, J. M. (2020). Transcriptional Patterns of Biogeochemically Relevant Marker Genes by Temperate Marine Bacteria. Frontiers in Microbiology, 11. doi:10.3389/fmicb.2020.00465
SponsorsWe are grateful to the crew of RV “José de Rioja” and colleagues from IEO Gijón/Xixón for their help with the RNA samples collection, especially to N. Arandia-Gorostidi and T. M. Huete-Stauffer. Bioinformatics analyses were supported by CESGA (Centro de Supercomputación de Galicia).
PublisherFrontiers Media SA
JournalFrontiers in microbiology
PubMed Central IDPMC7098952
Except where otherwise noted, this item's license is described as Archived with thanks to Frontiers in microbiology
- Expression patterns of elemental cycling genes in the Amazon River Plume.
- Authors: Satinsky BM, Smith CB, Sharma S, Landa M, Medeiros PM, Coles VJ, Yager PL, Crump BC, Moran MA
- Issue date: 2017 Aug
- Methanogens Are Major Contributors to Nitrogen Fixation in Soils of the Florida Everglades.
- Authors: Bae HS, Morrison E, Chanton JP, Ogram A
- Issue date: 2018 Apr 1
- Patterns of Transcript Abundance of Eukaryotic Biogeochemically-Relevant Genes in the Amazon River Plume.
- Authors: Zielinski BL, Allen AE, Carpenter EJ, Coles VJ, Crump BC, Doherty M, Foster RA, Goes JI, Gomes HR, Hood RR, McCrow JP, Montoya JP, Moustafa A, Satinsky BM, Sharma S, Smith CB, Yager PL, Paul JH
- Issue date: 2016
- Diel gene expression profiles of a phosphorus limited mountain lake using metatranscriptomics.
- Authors: Vila-Costa M, Sharma S, Moran MA, Casamayor EO
- Issue date: 2013 Apr
- Nutrient and temperature limitation of bacterioplankton growth in temperate lakes.
- Authors: Vrede K
- Issue date: 2005 Feb