Genetic tool development in marine protists: emerging model organisms for experimental cell biology
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Faktorová, Drahomíra
Nisbet, R. Ellen R.

Fernández Robledo, José A.
Casacuberta, Elena
Sudek, Lisa
Allen, Andrew E.

Ares, Manuel

Aresté, Cristina
Balestreri, Cecilia
Barbrook, Adrian C.
Beardslee, Patrick
Bender, Sara
Booth, David S.
Bouget, François-Yves
Bowler, Chris

Breglia, Susana A.
Brownlee, Colin
Burger, Gertraud

Cerutti, Heriberto
Cesaroni, Rachele
Chiurillo, Miguel A.
Clemente, Thomas
Coles, Duncan B.
Collier, Jackie L.

Cooney, Elizabeth C.

Coyne, Kathryn
Docampo, Roberto

Dupont, Christopher L.

Edgcomb, Virginia

Einarsson, Elin
Elustondo, Pía A.
Federici, Fernan
Freire-Beneitez, Veronica
Freyria, Nastasia J.

Fukuda, Kodai
García, Paulo A.
Girguis, Peter R.
Gomaa, Fatma
Gornik, Sebastian G.

Guo, Jian
Hampl, Vladimír
Hanawa, Yutaka
Haro-Contreras, Esteban R.
Hehenberger, Elisabeth
Highfield, Andrea
Hirakawa, Yoshihisa
Hopes, Amanda
Howe, Christopher J.

Hu, Ian

Ibañez, Jorge
Irwin, Nicholas A. T.
Ishii, Yuu

Janowicz, Natalia Ewa
Jones, Adam C.

Kachale, Ambar
Fujimura-Kamada, Konomi
Kaur, Binnypreet
Kaye, Jonathan Z.
Kazana, Eleanna
Keeling, Patrick J.
King, Nicole
Klobutcher, Lawrence A.
Lander, Noelia
Lassadi, Imen
Li, Zhuhong
Lin, Senjie

Lozano, Jean-Claude
Luan, Fulei
Maruyama, Shinichiro

Matute, Tamara
Miceli, Cristina
Minagawa, Jun

Moosburner, Mark
Najle, Sebastián R.

Nanjappa, Deepak
Nimmo, Isabel C.
Noble, Luke
Novák Vanclová, Anna M. G.
Nowacki, Mariusz

Nuñez, Isaac
Pain, Arnab

Piersanti, Angela

Pucciarelli, Sandra
Pyrih, Jan
Rest, Joshua S.
Rius, Mariana
Robertson, Deborah
Ruaud, Albane
Ruiz-Trillo, Iñaki
Sigg, Monika A.
Silver, Pamela A.

Slamovits, Claudio H.
Jason Smith, G.

Sprecher, Brittany N.

Stern, Rowena
Swart, Estienne C.
Tsaousis, Anastasios D.

Tsypin, Lev

Turkewitz, Aaron

Turnšek, Jernej

Valach, Matus

Vergé, Valérie
von Dassow, Peter
von der Haar, Tobias

Waller, Ross F.
Wang, Lu
Wen, Xiaoxue
Wheeler, Glen
Woods, April
Zhang, Huan
Mock, Thomas

Worden, Alexandra Z.

Lukeš, Julius

KAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionBioscience Program
Pathogen Genomics Laboratory
Date
2020-04-06Online Publication Date
2020-04-06Print Publication Date
2020-05Submitted Date
2019-07-26Permanent link to this record
http://hdl.handle.net/10754/662478
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Show full item recordAbstract
Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant taxa. Here, we report on the development of genetic tools in a range of protists primarily from marine environments. We present evidence for foreign DNA delivery and expression in 13 species never before transformed and for advancement of tools for eight other species, as well as potential reasons for why transformation of yet another 17 species tested was not achieved. Our resource in genetic manipulation will provide insights into the ancestral eukaryotic lifeforms, general eukaryote cell biology, protein diversification and the evolution of cellular pathways.Citation
Faktorová, D., Nisbet, R. E. R., Fernández Robledo, J. A., Casacuberta, E., Sudek, L., Allen, A. E., … Barbrook, A. C. (2020). Genetic tool development in marine protists: emerging model organisms for experimental cell biology. Nature Methods. doi:10.1038/s41592-020-0796-xSponsors
We thank M. Salisbury and D. Lacono, C. Poirier, M. Hamilton, C. Eckmann, H. Igel, C. Yung and K. Hoadley for assistance; V.K. Nagarajan, M. Accerbi and P.J. Green who carried out Agrobacterium studies in Heterosigma akashiwo, and N. Kraeva, C. Bianchi and V. Yurchenko for the help with designing the p57-V5+NeoR construct. We are also grateful to the protocols.io team (L. Teytelman and A. Broellochs) for their support. This collaborative effort was supported by the Gordon and Betty Moore Foundation EMS Program of the Marine Microbiology Initiative (grant nos. GBMF4972 and 4972.01 to F.-Y.B.; GBMF4970 and 4970.01 to M.A. and A.Z.W.; GBMF3788 to A.Z.W.; GBMF 4968 and 4968.01 to H.C.; GBMF4984 to V.H.; GBMF4974 and 4974.01 to C. Brownlee; GBMF4964 to Y. Hirakawa; GBMF4961 to T. Mock; GBMF4958 to P.S.; GBMF4957 to A.T.; GBMF4960 to G.J.S.; GBMF4979 to K.C.; GBMF4982 and 4982.01 to J.L.C.; GBMF4964 to P.J.K.; GBMF4981 to P.v.D.; GBMF5006 to A.E.A.; GBMF4986 to C.M.; GBMF4962 to J.A.F.R.; GBMF4980 and 4980.01 to S.L.; GBMF 4977 and 4977.01 to R.F.W.; GBMF4962.01 to C.H.S.; GBMF4985 to J.M.; GBMF4976 and 4976.01 to C.H.; GBMF4963 and 4963.01 to V.E.; GBMF5007 to C.L.D.; GBMF4983 and 4983.01 to J.L.; GBMF4975 and 4975.01 to A.D.T.; GBMF4973 and 4973.01 to I.R.-T. and GBMF4965 to N.K.), by The Leverhulme Trust (RPG-2017-364) to T. Mock and A. Hopes, and by ERD funds (16_019/0000759) from the Czech Ministry of Education to J.L.Publisher
Springer NatureJournal
Nature MethodsPubMed ID
32251396Additional Links
http://www.nature.com/articles/s41592-020-0796-xhttps://authors.library.caltech.edu/97621/5/41592_2020_796_MOESM1_ESM.pdf
ae974a485f413a2113503eed53cd6c53
10.1038/s41592-020-0796-x
Scopus Count
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