Electroactive biofilms on surface functionalized anodes: the anode respiring behavior of a novel electroactive bacterium, Desulfuromonas acetexigens
Conghaile, Peter Ó
KAUST DepartmentAli I. Al-Naimi Petroleum Engineering Research Center (ANPERC)
Biological and Environmental Sciences and Engineering (BESE) Division
Environmental Biotechnology Research Group
Environmental Science and Engineering Program
Physical Science and Engineering (PSE) Division
Water Desalination and Reuse Research Center (WDRC)
Online Publication Date2020-08-07
Print Publication Date2020-10
Permanent link to this recordhttp://hdl.handle.net/10754/662282
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Abstracturface chemistry is known to influence the formation, composition, and electroactivity of electron-conducting biofilms. However, understanding of the evolution of microbial composition during biofilm development and its impact on the electrochemical response is limited. Here we present voltammetric, microscopic and microbial community analysis of biofilms formed under fixed applied potential for modified graphite electrodes during early (90 h) and mature (340 h) growth phases. Electrodes modified to introduce hydrophilic groups (-NH2, -COOH and -OH) enhance early-stage biofilm formation compared to unmodified or electrodes modified with hydrophobic groups (-C2H5). In addition, early-stage films formed on hydrophilic electrodes were dominated by the gram-negative sulfur-reducing bacterium Desulfuromonas acetexigens while Geobacter sp. dominated on -C2H5 and unmodified electrodes. As biofilms mature, current generation becomes similar, and D. acetexigens dominates in all biofilms irrespective of surface chemistry. Electrochemistry of pure culture D. acetexigens biofilms reveal that this microbe is capable of forming electroactive biofilms producing considerable current density of > 9 A/m2 in a short period of potential induced growth (∼19 h followed by inoculation) using acetate as an electron donor. The inability of D. acetexigens biofilms to use H2 as a sole source electron donor for current generation shows promise for maximizing H2 recovery in single-chambered microbial electrolysis cell systems treating wastewaters.
CitationKaturi, K. P., Kamireddy, S., Kavanagh, P., Muhammad, A., Conghaile, P. Ó., Kumar, A., … Leech, D. (2020). Electroactive biofilms on surface functionalized anodes: the anode respiring behavior of a novel electroactive bacterium, Desulfuromonas acetexigens. Water Research, 116284. doi:10.1016/j.watres.2020.116284
Sponsorshis work was supported by the Center Competitive Funding Program (Grant No. FCC/1/1971-05-01) and the Competitive Research Grant (URF/1/2985-01-01) from King Abdullah University of Science and Technology (KAUST) and by a Charles Parsons Energy Research Award, through Science Foundation Ireland.