• Login
    View Item 
    •   Home
    • Research
    • Articles
    • View Item
    •   Home
    • Research
    • Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of KAUSTCommunitiesIssue DateSubmit DateThis CollectionIssue DateSubmit Date

    My Account

    Login

    Quick Links

    Open Access PolicyORCID LibguidePlumX LibguideSubmit an Item

    Statistics

    Display statistics

    Chimeric symbionts expressing a Wolbachia protein stimulate mosquito immunity and inhibit filarial parasite development

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    s42003-020-0835-2.pdf
    Size:
    1.016Mb
    Format:
    PDF
    Description:
    Published version
    Download
    Type
    Article
    Authors
    Epis, Sara cc
    Varotto-Boccazzi, Ilaria cc
    Crotti, Elena
    Damiani, Claudia
    Giovati, Laura cc
    Mandrioli, Mauro cc
    Biggiogera, Marco cc
    Gabrieli, Paolo
    Genchi, Marco
    Polonelli, Luciano
    Daffonchio, Daniele cc
    Favia, Guido
    Bandi, Claudio cc
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Bioscience Program
    Extreme Systems Microbiology Lab
    Date
    2020-03-06
    Online Publication Date
    2020-03-06
    Print Publication Date
    2020-12
    Submitted Date
    2019-12-24
    Permanent link to this record
    http://hdl.handle.net/10754/661952
    
    Metadata
    Show full item record
    Abstract
    Wolbachia can reduce the capability of mosquitoes to transmit infectious diseases to humans and is currently exploited in campaigns for the control of arboviruses, like dengue and Zika. Under the assumption that Wolbachia-mediated activation of insect immunity plays a role in the reduction of mosquito vectorial capacity, we focused our attention on the Wolbachia surface protein (WSP), a potential inductor of innate immunity. We hypothesized that the heterologous expression of this protein in gut- and tissue-associated symbionts may reduce parasite transmission. We thus engineered the mosquito bacterial symbiont Asaia to express WSP (AsaiaWSP). AsaiaWSP induced activation of the host immune response in Aedes aegypti and Anopheles stephensi mosquitoes, and inhibited the development of the heartworm parasite Dirofilaria immitis in Ae. aegypti. These results consolidate previous evidence on the immune-stimulating property of WSP and make AsaiaWSP worth of further investigations as a potential tool for the control of mosquito-borne diseases.
    Citation
    Epis, S., Varotto-Boccazzi, I., Crotti, E., Damiani, C., Giovati, L., Mandrioli, M., … Bandi, C. (2020). Chimeric symbionts expressing a Wolbachia protein stimulate mosquito immunity and inhibit filarial parasite development. Communications Biology, 3(1). doi:10.1038/s42003-020-0835-2
    Sponsors
    This study was supported by Cariplo Foundation and Lombardy Region to S.E. (2017-N.1656), by Transition Grant 2015-2017-Horizon 2020 to S.E. (G42F17000140001) and by the MIUR PRIN (Italian Ministry of Education, University and Research, MIUR) to C.B., L.P. and G.F. D.D. acknowledges the baseline funding support by King Abdullah University of Science and Technology (KAUST); S.E., C.B. and M.B. thank “Fondo per il finanziamento dei Dipartimenti universitari di eccellenza” (MIUR) of the Dept. of Biosciences (University of Milan) and of the Dept. of Biology and Biotechnology “L. Spallanzani” (University of Pavia). The authors thank Prof. L. Sacchi, Prof. F. Forlani and Prof. C. Bazzocchi for their suggestions and the UNITECH platform for the microscope image acquisition.
    Publisher
    Springer Science and Business Media LLC
    Journal
    Communications Biology
    DOI
    10.1038/s42003-020-0835-2
    Additional Links
    http://www.nature.com/articles/s42003-020-0835-2
    ae974a485f413a2113503eed53cd6c53
    10.1038/s42003-020-0835-2
    Scopus Count
    Collections
    Articles; Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program

    entitlement

     
    DSpace software copyright © 2002-2021  DuraSpace
    Quick Guide | Contact Us | Send Feedback
    Open Repository is a service hosted by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items. For anonymous users the allowed maximum amount is 50 search results.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.