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dc.contributor.authorTretina, Kyle
dc.contributor.authorHaidar, Malak
dc.contributor.authorMadsen-Bouterse, Sally A.
dc.contributor.authorSakura, Takaya
dc.contributor.authorMfarrej, Sara
dc.contributor.authorFry, Lindsay
dc.contributor.authorChaussepied, Marie
dc.contributor.authorPain, Arnab
dc.contributor.authorKnowles, Donald P.
dc.contributor.authorNene, Vishvanath M.
dc.contributor.authorGinsberg, Doron
dc.contributor.authorDaubenberger, Claudia A.
dc.contributor.authorBishop, Richard P.
dc.contributor.authorLangsley, Gordon
dc.contributor.authorSilva, Joana C.
dc.identifier.citationTretina, K., Haidar, M., Madsen-Bouterse, S. A., Sakura, T., Mfarrej, S., Fry, L., … Silva, J. C. (2020). Theileria parasites subvert E2F signaling to stimulate leukocyte proliferation. Scientific Reports, 10(1). doi:10.1038/s41598-020-60939-x
dc.description.abstractIntracellular pathogens have evolved intricate mechanisms to subvert host cell signaling pathways and ensure their own propagation. A lineage of the protozoan parasite genus Theileria infects bovine leukocytes and induces their uncontrolled proliferation causing a leukemia-like disease. Given the importance of E2F transcription factors in mammalian cell cycle regulation, we investigated the role of E2F signaling in Theileria-induced host cell proliferation. Using comparative genomics and surface plasmon resonance, we identified parasite-derived peptides that have the sequence-specific ability to increase E2F signaling by binding E2F negative regulator Retinoblastoma-1 (RB). Using these peptides as a tool to probe host E2F signaling, we show that the disruption of RB complexes ex vivo leads to activation of E2F-driven transcription and increased leukocyte proliferation in an infection-dependent manner. This result is consistent with existing models and, together, they support a critical role of E2F signaling for Theileria-induced host cell proliferation, and its potential direct manipulation by one or more parasite proteins.
dc.description.sponsorshipWe are grateful to Dr. Bret A. Hassel for manuscript feedback, and Linda Hamburg for her contributions to the mouse and laboratory work. This work was supported in part by the United States Department of Agriculture, Agricultural Research Service (USDA-ARS) (58-5348-4-013), by the Norman Borlaug Commemorative Research Initiative, an initiative between the Feed the Future program of the U.S. Agency for International Development (USAID) and USDA-ARS (58-5348-2-117 F), by the Department for International Development of the United Kingdom, by the Bill and Melinda Gates Foundation (OPP1078791), and by the National Institute of Allergy and Infectious Diseases (T32 AI007540-14, on Immunity and Infection). This work was also supported by the grant Labex ParaFrap (ANR-11-LABX-0024) and core funding from INSERM and the CNRS awarded to GL and a CRG4 grant (URF/1/2610-01-01) from the Office for Sponsored Research (OSR) in King Abdullah University of Science and Technology (KAUST) award to AP and GL and the faculty baseline fund (BAS/1/1020-01-01) awarded to AP.
dc.publisherSpringer Nature
dc.rightsArchived with thanks to Scientific Reports
dc.titleTheileria parasites subvert E2F signaling to stimulate leukocyte proliferation
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentPathogen Genomics Laboratory
dc.identifier.journalScientific Reports
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionInstitute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD, 21201, USA.
dc.contributor.institutionLaboratoire de Biologie Comparative des Apicomplexes, Faculté de Médicine, Université Paris Descartes, Sorbonne, Paris Cité, France.
dc.contributor.institutionDepartment of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, 99164-7040, USA.
dc.contributor.institutionInternational Livestock Research Institute, Nairobi, 00100, Kenya.
dc.contributor.institutionWeizmann Institute of Science, Molecular Cell Biology Department, PO Box 26, Rehovot, 76100, Israel.
dc.contributor.institutionSwiss Tropical and Public Health Institute, Basel, Switzerland.
kaust.personMfarrej, Sara
kaust.personPain, Arnab
kaust.acknowledged.supportUnitSponsored Research

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