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dc.contributor.authorBi, Chongwei
dc.contributor.authorWang, Lin
dc.contributor.authorYuan, Baolei
dc.contributor.authorZhou, Xuan
dc.contributor.authorLi, Yu
dc.contributor.authorWang, Sheng
dc.contributor.authorPang, Yuhong
dc.contributor.authorGao, Xin
dc.contributor.authorHuang, Yanyi
dc.contributor.authorLi, Mo
dc.date.accessioned2020-08-25T06:09:25Z
dc.date.available2020-02-19T12:14:43Z
dc.date.available2020-08-25T06:09:25Z
dc.date.issued2020-08-24
dc.date.submitted2020-03-13
dc.identifier.citationBi, C., Wang, L., Yuan, B., Zhou, X., Li, Y., Wang, S., … Li, M. (2020). Long-read individual-molecule sequencing reveals CRISPR-induced genetic heterogeneity in human ESCs. Genome Biology, 21(1). doi:10.1186/s13059-020-02143-8
dc.identifier.issn1474-760X
dc.identifier.doi10.1186/s13059-020-02143-8
dc.identifier.doi10.1101/2020.02.10.942151
dc.identifier.urihttp://hdl.handle.net/10754/661565
dc.description.abstractQuantifying the genetic heterogeneity of a cell population is essential to understanding of biological systems. We develop a universal method to label individual DNA molecules for single-base-resolution haplotype-resolved quantitative characterization of diverse types of rare variants, with frequency as low as 4 × 10−5 , using both short- or long-read sequencing platforms. It provides the first quantitative evidence of persistent nonrandom large structural variants and an increase in singlenucleotide variants at the on-target locus following repair of double-strand breaks induced by CRISPR-Cas9 in human embryonic stem cells.
dc.publisherSpringer Nature
dc.relation.urlhttps://genomebiology.biomedcentral.com/articles/10.1186/s13059-020-02143-8
dc.rightsThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleLong-read individual-molecule sequencing reveals CRISPR-induced genetic heterogeneity in human ESCs
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.contributor.departmentComputer Science
dc.contributor.departmentComputer Science Program
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
dc.contributor.departmentLaboratory of Stem Cell and Regeneration, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Kingdom of Saudi Arabia.
dc.contributor.departmentStructural and Functional Bioinformatics Group
dc.identifier.journalGenome Biology
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionPresent address: Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, China.
dc.contributor.institutionBeijing Advanced Innovation Center for Genomics (ICG), Biomedical Pioneering Innovation Center (BIOPIC), School of Life Sciences, College of Chemistry, College of Engineering, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China.
dc.contributor.institutionInstitute for Cell Analysis, Shenzhen Bay Laboratory, Shenzhen, China.
dc.identifier.volume21
dc.identifier.issue1
kaust.personBi, Chongwei
kaust.personWang, Lin
kaust.personYuan, Baolei
kaust.personZhou, Xuan
kaust.personLi, Yu
kaust.personWang, Sheng
kaust.personGao, Xin
kaust.personLi, Mo
kaust.grant.numberURF/1/3412-01-01)
kaust.grant.numberBAS/1/1080-01
dc.date.accepted2020-08-11
dc.relation.issupplementedbyDOI:10.6084/m9.figshare.c.5100908
dc.relation.issupplementedbybioproject:PRJNA606194
dc.relation.issupplementedbyDOI:10.5281/zenodo.3977107
refterms.dateFOA2020-02-19T12:15:54Z
display.relations<b>Is Supplemented By:</b><br/> <ul><li><i>[Dataset]</i> <br/> Chongwei Bi, Wang, L., Baolei Yuan, Zhou, X., Li, Y., Wang, S., Yuhong Pang, Gao, X., Yanyi Huang, &amp; Li, M. (2020). Long-read individual-molecule sequencing reveals CRISPR-induced genetic heterogeneity in human ESCs. figshare. https://doi.org/10.6084/M9.FIGSHARE.C.5100908. DOI: <a href="https://doi.org/10.6084/m9.figshare.c.5100908" >10.6084/m9.figshare.c.5100908</a> Handle: <a href="http://hdl.handle.net/10754/664976" >10754/664976</a></a></li><li><i>[Bioproject]</i> <br/> Title: Individual-molecule Sequencing (IDMseq) of PANX1 gene in hESCs. Publication Date: 2020-02-12. bioproject: <a href="https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA606194" >PRJNA606194</a> Handle: <a href="http://hdl.handle.net/10754/666916" >10754/666916</a></a></li><li><i>[Software]</i> <br/> Bi, C. (2020). <i>Variant Analysis with UMI for Long-read Technology (VAULT)</i> (Version v0.3) [Computer software]. Zenodo. https://doi.org/10.5281/ZENODO.3977107. DOI: <a href="https://doi.org/10.5281/zenodo.3977107" >10.5281/zenodo.3977107</a> Handle: <a href="http://hdl.handle.net/10754/667908" >10754/667908</a></a></li></ul>
kaust.acknowledged.supportUnitOSR
dc.date.published-online2020-08-24
dc.date.published-print2020-12


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This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
Except where otherwise noted, this item's license is described as This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
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