Metal self-assembly mimosine peptides with enhanced antimicrobial activity: towards a new generation of multitasking chelating agents.
AuthorsLachowicz, Joanna Izabela Izabela
Dalla Torre, Gabriele
Nurchi, Valeria Marina
Bachor, Remigiusz RB
Pisano, Maria Barbara MBP
Cosentino, Sofia SC
Ibba, Antonella AI
Mujika, Joni JM
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Embargo End Date2021-01-28
Permanent link to this recordhttp://hdl.handle.net/10754/661514
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AbstractMimosine is a non-protein aminoacid with various properties, such as antibacterial, anti-inflammatory, anti-cancer and anti-virus among others. Due to its structure similarity with deferiprone (DFP), mimosine is a potential excellent metal chelator. In the present work, we combine experimental and theoretical (DFT) approaches in order to investigate the properties of mimosine peptides. Six different peptides were synthesized and their complex stoichiometry and stability were characterized by means of UV-Vis spectrophotometry. Then, the binding mode and self-assembly features of the peptides were evaluated using a DFT approach, taking into account different number of mimosine amino acids and varying the length of the spacer between the mimosine residues, finding a good agreement between experimental data and computational calculations. Further elucidations of the structural properties of these peptides allowed us to propose improvements in the structure of the mimosine moiety that can lead to enhanced affinity for high-valent metals. Moreover, we demonstrate that these peptides show an anti-microbial activity against gram positive bacteria that is enhanced by the formation of a complex with iron(III) ions. The mimosine peptides could be an alternative to Antimicrobial peptides (AMPs), which are expensive and susceptible to proteolytic degradation. In summary, in the present work, we propose a new generation of multipurpose mimosine-based peptides as a new metal self-assembly chelators that could be a corner point in biomedical and nanotechnological applications.
CitationLachowicz, J. I. I., Dalla Torre, G., Cappai, R., Randaccio, E., Nurchi, V. M., Bachor, R. R., … Lopez, X. (2020). Metal self-assembly mimosine peptides with enhanced antimicrobial activity: towards a new generation of multitasking chelating agents. Dalton Transactions. doi:10.1039/c9dt04545g
SponsorsThis work was supported by a grant No. UMO-2015/17/D/ST5/01329 from the National Science Centre, Poland. This work was also supported by Grants PGC2018-099321-B-I00 from the Ministry of Science and Universities through the Office of Science Research (MINECO/FEDER), and Grant IT588-13 from the Basque Government.
PublisherRoyal Society of Chemistry (RSC)