Biomembrane-based organic electronic devices for ligand-receptor binding studies.
Type
ArticleAuthors
Liu, Han-YuanPappa, Anna-Maria
Hidalgo, Tania Cecilia
Inal, Sahika
Owens, Rόisín M
Daniel, Susan
KAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionBioscience Program
Date
2020-02-05Online Publication Date
2020-02-05Print Publication Date
2020-09Submitted Date
2019-12-02Permanent link to this record
http://hdl.handle.net/10754/661484Metadata
Show full item recordAbstract
We present a simple, rapid method for forming supported lipid bilayers on organic electronic devices composed of conducting polymer electrodes using a solvent-assisted lipid bilayer formation method. These supported bilayers present protein recognition elements that are mobile, critical for multivalent binding interactions. Because these polymers are transparent and conducting, we demonstrate, by optical and electrical detection, the specific interactions of proteins with these biomembrane-based bioelectronic devices. This work paves the way for easy formation of biomembrane mimetics for sensing and detection of binding events in a label-free manner on organic electronic devices of more sophisticated architectures. Graphical abstract.Citation
Liu, H.-Y., Pappa, A.-M., Hidalgo, T. C., Inal, S., Owens, R. M., & Daniel, S. (2020). Biomembrane-based organic electronic devices for ligand–receptor binding studies. Analytical and Bioanalytical Chemistry. doi:10.1007/s00216-020-02449-3Sponsors
We thank Professor Nam-Joon Cho (the Engineering in Translational Science Group at Nanyang Technical University) for his advice and providing the microfluidic flow cells used in this work. The views and conclusions contained in this document are those of the authors and should not be interpreted as representing the official policies, either expressed or implied, of DARPA or the Army Research Office or the U.S.Government.A.M.P. received funding from the Oppenheimer Junior Research Fellowship. Part of this work was supported by the King Abdullah University of Science and Technology (KAUST) Office of Sponsored Research (OSR) under Award No. OSR-2018-CRG7-3709. This research was sponsored in part by the Defense Advanced Research Projects Agency (DARPA) Army Research Office and was accomplished under Cooperative agreement number W911NF-18-2-0152.