Complete Genome Sequence of Paenibacillus sp. JZ16, a Plant Growth Promoting Root Endophytic Bacterium of the Desert Halophyte Zygophyllum Simplex.
Biological and Environmental Sciences and Engineering (BESE) Division
Computational Bioscience Research Center (CBRC)
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Desert Agriculture Initiative
Online Publication Date2020-02-03
Print Publication Date2020-06
Embargo End Date2021-02-05
Permanent link to this recordhttp://hdl.handle.net/10754/661478
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AbstractPaenibacillus sp. JZ16 is a gram-positive, rod-shaped, motile root endophytic bacterium of the pioneer desert halophytic plant Zygophyllum simplex. JZ16 was previously shown to promote salinity stress tolerance in Arabidopsis thaliana and possesses a highly motile phenotype on nutrient agar. JZ16 genome sequencing using PacBio generated 82,236 reads with a mean insert read length of 11,432 bp and an estimated genome coverage of 127X, resulting in a chromosome of 7,421,843 bp with a GC content of 49.25% encoding 6710 proteins, 8 rRNA operons, 117 ncRNAs and 73 tRNAs. Whole-genome sequencing analysis revealed a potentially new species for JZ16. Functional analysis revealed the presence of a number of enzymes involved in the breakdown of plant-based polymers. JZ16 could be of potential use in agricultural applications for promoting biotic and abiotic stress tolerance and for biotechnological processes (e.g., as biocatalysts for biofuel production). The culture-dependent collection of bacterial endophytes from desert plants combined with genome sequence mining provides new opportunities for industrial applications.
CitationEida, A. A., Bougouffa, S., Alam, I., Hirt, H., & Saad, M. M. (2020). Complete Genome Sequence of Paenibacillus sp. JZ16, a Plant Growth Promoting Root Endophytic Bacterium of the Desert Halophyte Zygophyllum Simplex. Current Microbiology. doi:10.1007/s00284-020-01908-5
SponsorsThe work presented is part of the DARWIN21 project (https://www.darwin21.org/), with the objective to improve sustainable agriculture on arid lands by exploiting microbes isolated from pioneer desert plants that are able to survive in extreme environmental conditions. The authors would thank all members of Hirt lab, CDA management team and the Bioscience Core Labs in KAUST for the technical assistance and for their help in many aspects of this work.
The work was funded by KAUST baseline research project BAS/1/1062–01-01 of H.H., the authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest
PublisherSpringer Science and Business Media LLC
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