Performance of extraction methods for extracellular DNA from sediments across marine habitats
Type
ArticleKAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionMarine Science Program
Red Sea Research Center (RSRC)
Red Sea Research Center and Computational Biosciences Research Center King Abdullah University of Science and Technology (KAUST) Thuwal Saudi Arabia
Date
2019-11-02Online Publication Date
2019-11-02Print Publication Date
2020-01Submitted Date
2019-07-25Permanent link to this record
http://hdl.handle.net/10754/661405
Metadata
Show full item recordAbstract
Using environmental DNA (eDNA) to detect taxa is quickly becoming an important technique to determine the distribution and diversity of macroorganisms. Assessing differences in communities based on eDNA extracted from sediment could enhance our understanding of marine communities among the many different marine habitats both from the present and from the past using sediment cores. Although the effect of extraction methods on metabarcoding results has been tested for both water and sediment samples, less is known about how sediment types may affect the extraction and sequencing of eDNA.Citation
Geraldi, N. R., Díaz-Rúa, R., Shea, L. A., & Duarte, C. M. (2019). Performance of extraction methods for extracellular DNA from sediments across marine habitats. Environmental DNA, 2(1), 91–98. doi:10.1002/edn3.48Sponsors
This research was supported by the King Abdullah University of Science and Technology through baseline funding and funds by the Tarek Ahmed Juffali Research Chair in Red Sea Ecology. We thank A. Anton, P. Carrillo de Albornoz, and J. Martinez Ayala for help-ing to collect samples. All authors declare they have not conflict of interest.Publisher
WileyJournal
Environmental DNADOI
10.1002/edn3.48Additional Links
https://onlinelibrary.wiley.com/doi/abs/10.1002/edn3.48https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/edn3.48
Relations
Is Supplemented By:- [Bioproject]
Title: Performance of extraction methods for extracellular DNA from sediments across marine habitatsPublication Date: 2019-10-23. bioproject: PRJNA579138 Handle: 10754/666534
ae974a485f413a2113503eed53cd6c53
10.1002/edn3.48
Scopus Count
Except where otherwise noted, this item's license is described as This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.