Performance of extraction methods for extracellular DNA from sediments across marine habitats
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Marine Science Program
Red Sea Research Center (RSRC)
Red Sea Research Center and Computational Biosciences Research Center King Abdullah University of Science and Technology (KAUST) Thuwal Saudi Arabia
Online Publication Date2019-11-02
Print Publication Date2020-01
Permanent link to this recordhttp://hdl.handle.net/10754/661405
MetadataShow full item record
AbstractUsing environmental DNA (eDNA) to detect taxa is quickly becoming an important technique to determine the distribution and diversity of macroorganisms. Assessing differences in communities based on eDNA extracted from sediment could enhance our understanding of marine communities among the many different marine habitats both from the present and from the past using sediment cores. Although the effect of extraction methods on metabarcoding results has been tested for both water and sediment samples, less is known about how sediment types may affect the extraction and sequencing of eDNA.
CitationGeraldi, N. R., Díaz-Rúa, R., Shea, L. A., & Duarte, C. M. (2019). Performance of extraction methods for extracellular DNA from sediments across marine habitats. Environmental DNA, 2(1), 91–98. doi:10.1002/edn3.48
SponsorsThis research was supported by the King Abdullah University of Science and Technology through baseline funding and funds by the Tarek Ahmed Juffali Research Chair in Red Sea Ecology. We thank A. Anton, P. Carrillo de Albornoz, and J. Martinez Ayala for help-ing to collect samples. All authors declare they have not conflict of interest.
RelationsIs Supplemented By:
Except where otherwise noted, this item's license is described as This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.