Characterization of a sponge microbiome using an integrative genome-centric approach
AuthorsEngelberts, J. Pamela
Robbins, Steven J.
de Goeij, Jasper M.
Bell, Sara C.
Webster, Nicole S.
KAUST DepartmentMarine Science Program
Red Sea Research Center (RSRC)
Biological and Environmental Sciences and Engineering (BESE) Division
Online Publication Date2020-01-28
Print Publication Date2020-05
Embargo End Date2020-07-28
Permanent link to this recordhttp://hdl.handle.net/10754/661330
MetadataShow full item record
AbstractMarine sponges often host diverse and species-specific communities of microorganisms that are critical for host health. Previous functional genomic investigations of the sponge microbiome have focused primarily on specific symbiont lineages, which frequently make up only a small fraction of the overall community. Here, we undertook genome-centric analysis of the symbiont community in the model species Ircinia ramosa and analyzed 259 unique, high-quality metagenomeassembled genomes (MAGs) that comprised 74% of the I. ramosa microbiome. Addition of these MAGs to genome trees containing all publicly available microbial sponge symbionts increased phylogenetic diversity by 32% within the archaea and 41% within the bacteria. Metabolic reconstruction of the MAGs showed extensive redundancy across taxa for pathways involved in carbon fixation, B-vitamin synthesis, taurine metabolism, sulfite oxidation, and most steps of nitrogen metabolism. Through the acquisition of all major taxa present within the I. ramosa microbiome, we were able to analyze the functional potential of a sponge-associated microbial community in unprecedented detail. Critical functions, such as carbon fixation, which had previously only been assigned to a restricted set of sponge-associated organisms, were actually spread across diverse symbiont taxa, whereas other essential pathways, such as ammonia oxidation, were confined to specific keystone taxa.
CitationEngelberts, J. P., Robbins, S. J., de Goeij, J. M., Aranda, M., Bell, S. C., & Webster, N. S. (2020). Characterization of a sponge microbiome using an integrative genome-centric approach. The ISME Journal. doi:10.1038/s41396-020-0591-9
SponsorsWe acknowledge Andrea Severati and other staff at the Australian Institute of Marine Science SeaSim facility who assisted with sample collection and sponge husbandry.
JournalThe ISME Journal