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dc.contributor.authorOthoum, Ghofran K.
dc.contributor.authorPrigent, S.
dc.contributor.authorDerouiche, A.
dc.contributor.authorShi, L.
dc.contributor.authorBokhari, Ameerah
dc.contributor.authorAlamoudi, S.
dc.contributor.authorBougouffa, Salim
dc.contributor.authorGao, Xin
dc.contributor.authorHoehndorf, Robert
dc.contributor.authorArold, Stefan T.
dc.contributor.authorGojobori, Takashi
dc.contributor.authorHirt, Heribert
dc.contributor.authorLafi, Feras Fawzi
dc.contributor.authorNielsen, J.
dc.contributor.authorBajic, Vladimir B.
dc.contributor.authorMijakovic, I.
dc.contributor.authorEssack, Magbubah
dc.date.accessioned2019-12-30T06:52:36Z
dc.date.available2019-12-30T06:52:36Z
dc.date.issued2019-12-17
dc.identifier.citationOthoum, G., Prigent, S., Derouiche, A., Shi, L., Bokhari, A., Alamoudi, S., … Essack, M. (2019). Comparative genomics study reveals Red Sea Bacillus with characteristics associated with potential microbial cell factories (MCFs). Scientific Reports, 9(1). doi:10.1038/s41598-019-55726-2
dc.identifier.doi10.1038/s41598-019-55726-2
dc.identifier.urihttp://hdl.handle.net/10754/660865
dc.description.abstractRecent advancements in the use of microbial cells for scalable production of industrial enzymes encourage exploring new environments for efficient microbial cell factories (MCFs). Here, through a comparison study, ten newly sequenced Bacillus species, isolated from the Rabigh Harbor Lagoon on the Red Sea shoreline, were evaluated for their potential use as MCFs. Phylogenetic analysis of 40 representative genomes with phylogenetic relevance, including the ten Red Sea species, showed that the Red Sea species come from several colonization events and are not the result of a single colonization followed by speciation. Moreover, clustering reactions in reconstruct metabolic networks of these Bacillus species revealed that three metabolic clades do not fit the phylogenetic tree, a sign of convergent evolution of the metabolism of these species in response to special environmental adaptation. We further showed Red Sea strains Bacillus paralicheniformis (Bac48) and B. halosaccharovorans (Bac94) had twice as much secreted proteins than the model strain B. subtilis 168. Also, Bac94 was enriched with genes associated with the Tat and Sec protein secretion system and Bac48 has a hybrid PKS/NRPS cluster that is part of a horizontally transferred genomic region. These properties collectively hint towards the potential use of Red Sea Bacillus as efficient protein secreting microbial hosts, and that this characteristic of these strains may be a consequence of the unique ecological features of the isolation environment.
dc.description.sponsorshipTe authors wish to acknowledge the experimental support from the King Abdullah University of Science and Technology (KAUST) Bioscience Core Laboratory. Te research reported in this publication was supported by King Abdullah University of Science and Technology (KAUST) through the Awards Nos. FCC/1/1976-02-01, FCC/1/1976-17-01, FCC/1/1976-03-01, FCC/1/1976-20-01, FCC/1/1976-16-01, FCS/1/2911-01-01, BAS/1/1606- 01-01, URF/1/1976-06-01, BAS/1/1624-01-01, BAS/1/1659-01-01, BAS/1/1059-01-01 from the Office of Sponsored Research (OSR).
dc.publisherSpringer Nature
dc.relation.urlhttp://www.nature.com/articles/s41598-019-55726-2
dc.relation.urlhttps://www.nature.com/articles/s41598-019-55726-2.pdf
dc.rightsThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleComparative genomics study reveals Red Sea Bacillus with characteristics associated with potential microbial cell factories (MCFs)
dc.typeArticle
dc.contributor.departmentApplied Mathematics and Computational Science Program
dc.contributor.departmentBio-Ontology Research Group (BORG)
dc.contributor.departmentBioinformatics
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentChemical Engineering Program
dc.contributor.departmentChemical and Biological Engineering Program
dc.contributor.departmentCompetitive Research Funds
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.contributor.departmentComputer Science Program
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
dc.contributor.departmentDesert Agriculture Initiative
dc.contributor.departmentHirt Lab
dc.contributor.departmentOCRF- Special Academic Partnership
dc.contributor.departmentPlant Science
dc.contributor.departmentStructural Biology and Engineering
dc.contributor.departmentStructural and Functional Bioinformatics Group
dc.identifier.journalScientific Reports
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionDepartment of Biology and Biological Engineering, Division of Systems & Synthetic Biology, Chalmers University of Technology, Kemivägen 10, 41296, Gothenburg, Sweden.
dc.contributor.institutionDepartment of Biology, Science and Arts College, King Abdulaziz University, Rabigh, 21589, Kingdom of Saudi Arabia.
kaust.personOthoum, Ghofran K
kaust.personBokhari, Ameerah
kaust.personBougouffa, Salim
kaust.personGao, Xin
kaust.personHoehndorf, Robert
kaust.personArold, Stefan T.
kaust.personGojobori, Takashi
kaust.personHirt, Heribert
kaust.personLafi, Feras Fawzi
kaust.personBajic, Vladimir B.
kaust.personEssack, Magbubah
kaust.grant.numberBAS/1/1624-01-01
kaust.grant.numberFCC/1/1976-02-01
kaust.grant.numberFCC/1/1976-17-01
kaust.grant.numberFCS/1/2911-01-01
kaust.grant.numberURF/1/1976-06
refterms.dateFOA2019-12-30T06:53:08Z
kaust.acknowledged.supportUnitBioscience Core Laboratory
kaust.acknowledged.supportUnitOffice of Sponsored Research (OSR)
dc.date.published-online2019-12-17
dc.date.published-print2019-12


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This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
Except where otherwise noted, this item's license is described as This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.