Wounding and insect feeding trigger two independent MAPK pathways with distinct regulation and kinetics
Schenk, Sebastian T.
KAUST DepartmentPlant Science
Desert Agriculture Initiative
Biological and Environmental Sciences and Engineering (BESE) Division
Permanent link to this recordhttp://hdl.handle.net/10754/660715
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AbstractWounding is caused by abiotic and biotic factors and triggers complex short- and long-term responses at the local and systemic level. These responses are under the control of complex signaling pathways, which are still poorly understood. Here, we show that the rapid activation of MKK4/5-MPK3/6 by wounding is independent of jasmonic acid (JA) signaling and that, contrary to what happens in tobacco, this fast module does not control wound-triggered JA accumulation in Arabidopsis. We also demonstrate that a second MAPK module, constituted by MKK3 and the clade-C MAPKs MPK1/2/7, is activated by wounding in an independent manner. We provide evidence that the activation of this MKK3-MPK1/2/7 module occurs mainly through wound-induced JA production via the transcriptional regulation of upstream clade-III MAP3Ks and particularly MAP3K14. We show that mkk3 mutant plants are more susceptible to the larvae of the generalist lepidopteran herbivore Spodoptera littoralis, indicating that the MKK3-MPK1/2/7 module is involved in counteracting insect feeding.
CitationSözen, C., Schenk, S. T., Boudsocq, M., Chardin, C., Almeida-Trapp, M., Krapp, A., … Colcombet, J. (2019). Wounding and insect feeding trigger two independent MAPK pathways with distinct regulation and kinetics. doi:10.1101/855098
SponsorsC.S., H.H., and J.C. designed the research. C.S., S.T.S., M.B., C.C., A.K., M.A-T., A.M. and J.C. performed research. All the authors contributed to the manuscript. We thank the Stress Signaling group for critical discussion of this work. We also thank Shuqun Zhang and Edward Farmer for providing mkk4mkk5 and coi1-34 seeds. This work has benefited from a French State grant (LabEx Saclay Plant Sciences-SPS, ANR-10-LABX-0040-SPS), managed by the French National Research Agency under an “Investments for the Future” program (ANR-11-IDEX-0003-02). C.S. and C.C. were funded by SPS PhD fellowships. Marilia Almeida-Trapp gratefully acknowledges financial support by a Capes-Humboldt Research Fellowship. This publication has been written with the support of the AgreenSkills+ fellowship program which has received funding from the EU’s Seventh Framework Program under grant agreement No. FP7-609398 (AgreenSkills+ contract) to STS.
PublisherCold Spring Harbor Laboratory
Except where otherwise noted, this item's license is described as Archived with thanks to Cold Spring Harbor Laboratory