Identification of methanogenesis and syntrophy as important microbial metabolic processes for optimal thermophilic anaerobic digestion of energy cane thin stillage
AuthorsOosterkamp, Margreet J.
Ibáñez, Ana B.
Mackie, Roderick I.
KAUST DepartmentEnvironmental Science and Engineering Program
Water Desalination and Reuse Research Center (WDRC)
Biological and Environmental Sciences and Engineering (BESE) Division
Embargo End Date2020-06-01
Permanent link to this recordhttp://hdl.handle.net/10754/660583
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AbstractThe aim of this research was to identify key microorganisms for thermophilic (55 °C) anaerobic digestion of thin stillage derived from hydrolysis and ethanol fermentation of energy cane in a conventional stirred tank reactor with a 10-day hydraulic retention time. Efficient thermophilic anaerobic digestion with a specific methane production of 0.43 Lmethane/gtCOD used/d and biogas containing around 56% methane was accomplished. Due to an overnight temperature perturbation the specific methane production decreased to 0.16 Lmethane/gtCOD used/d. Analysis of the microbial community showed the importance of methanogenic Archaea belonging to Methanosarcina and Methanothermobacter as well as syntrophic Bacteria related to Thermacetogenium, Tepidanaerobacter and Anaerobaculum. This indicates that retention of biomass maintaining syntrophy and methanogenesis more efficiently may be useful for thermophilic anaerobic digestion of thin stillage derived from the production of energy cane ethanol.
CitationOosterkamp, M. J., Bauer, S., Ibáñez, A. B., Méndez-García, C., Hong, P.-Y., Cann, I., & Mackie, R. I. (2019). Identification of methanogenesis and syntrophy as important microbial metabolic processes for optimal thermophilic anaerobic digestion of energy cane thin stillage. Bioresource Technology Reports, 7, 100254. doi:10.1016/j.biteb.2019.100254
SponsorsWe greatly appreciate the help of Sabrina Zimmerman (BP Biofuels, University of California at Berkeley, Berkeley, CA, USA), Glen Austin (BP pilot plant, Jennings, LA, USA), Michael Harland and Robert Brown (School of Chemical Sciences, Machine Shop, University of Illinois, Urbana, IL, USA), Chris Wright and Álvaro Hernández (W.M. Keck Center, Roy J. Carver Biotechnology Center, University of Illinois, Urbana, IL, USA). This research was supported by the Energy Biosciences Institute (USA, project OO2J14).
JournalBioresource Technology Reports