Phosphorylation-dependent control of an RNA granule-localized protein that fine-tunes defence gene expression at a post-transcriptional level
Maldonado-Bonilla, Luis D.
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AbstractMitogen-activated protein kinase (MAPK) cascades are key signalling modules of plant defence responses to pathogen-associated molecular patterns [PAMPs; e.g. the bacterial peptide flagellin (flg22)]. Tandem zinc finger protein 9 (TZF9) is a RNA-binding protein that is phosphorylated by two PAMP-responsive MAPKs, MPK3 and MPK6. We mapped the major phosphosites in TZF9 and showed their importance for controlling in vitro RNA-binding activity, in vivo flg22-induced rapid disappearance of TZF9-labelled processing body-like structures and TZF9 protein turnover. Microarray analysis showed a strong discordance between transcriptome (total mRNA) and translatome (polysome-associated mRNA) in the tzf9 mutant, with more mRNAs associated with ribosomes in the absence of TZF9. This suggests that TZF9 may sequester and inhibit the translation of subsets of mRNAs. Fittingly, TZF9 physically interacts with poly(A)-binding protein 2 (PAB2), a hallmark constituent of stress granules – sites for stress-induced translational stalling/arrest. TZF9 even promotes the assembly of stress granules in the absence of stress. Hence, MAPKs may control defence gene expression post-transcriptionally through release from translation arrest within TZF9–PAB2-containing RNA granules or by perturbing the function of PAB2 in translation control (e.g. in the mRNA closed-loop model of translation).
CitationTabassum, N., Eschen-Lippold, L., Athmer, B., Baruah, M., Brode, M., Maldonado-Bonilla, L. D., … Lee, J. (2019). Phosphorylation-dependent control of an RNA granule-localized protein that fine-tunes defence gene expression at a post-transcriptional level. The Plant Journal. doi:10.1111/tpj.14573
SponsorsThis work was funded by German Research Foundation through the CRC648 programme (Molecular mechanisms of information processing in plants) and, in part, through core funding of the Leibniz Institute for Plant Biochemistry (IPB). MB was supported by a fellowship from the Erasmus Mundus BRAVE program. We thank Professor Alain Tissier (IPB) for providing the microarray chip-compatible GeneAltas® system facilities, Dr Stefan Bennewitz for advice and support in the microarray experiment, and Drs Katerina Bendak and Joey Mackey (University of Sydney) for the Pentaprobe plasmids. Many thanks to Ms Nicole Bauer and Ms Petra Majovsky for excellent technical assistance.
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