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dc.contributor.authorMahas, Ahmed
dc.contributor.authorAman, Rashid
dc.contributor.authorMahfouz, Magdy M.
dc.date.accessioned2019-12-12T07:04:42Z
dc.date.available2019-12-12T07:04:42Z
dc.date.issued2019-12-02
dc.identifier.citationMahas, A., Aman, R., & Mahfouz, M. (2019). CRISPR-Cas13d mediates robust RNA virus interference in plants. Genome Biology, 20(1). doi:10.1186/s13059-019-1881-2
dc.identifier.doi10.1186/s13059-019-1881-2
dc.identifier.urihttp://hdl.handle.net/10754/660537
dc.description.abstractBACKGROUND:CRISPR-Cas systems endow bacterial and archaeal species with adaptive immunity mechanisms to fend off invading phages and foreign genetic elements. CRISPR-Cas9 has been harnessed to confer virus interference against DNA viruses in eukaryotes, including plants. In addition, CRISPR-Cas13 systems have been used to target RNA viruses and the transcriptome in mammalian and plant cells. Recently, CRISPR-Cas13a has been shown to confer modest interference against RNA viruses. Here, we characterized a set of different Cas13 variants to identify those with the most efficient, robust, and specific interference activities against RNA viruses in planta using Nicotiana benthamiana. RESULTS:Our data show that LwaCas13a, PspCas13b, and CasRx variants mediate high interference activities against RNA viruses in transient assays. Moreover, CasRx mediated robust interference in both transient and stable overexpression assays when compared to the other variants tested. CasRx targets either one virus alone or two RNA viruses simultaneously, with robust interference efficiencies. In addition, CasRx exhibits strong specificity against the target virus and does not exhibit collateral activity in planta. CONCLUSIONS:Our data establish CasRx as the most robust Cas13 variant for RNA virus interference applications in planta and demonstrate its suitability for studying key questions relating to virus biology.
dc.description.sponsorshipWe wish to thank members of the Laboratory for Genome Engineering and Synthetic Biology at King Abdullah University of Science and Technology for helpful discussions and comments on the manuscript.
dc.publisherSpringer Science and Business Media LLC
dc.relation.urlhttps://genomebiology.biomedcentral.com/articles/10.1186/s13059-019-1881-2
dc.relation.urlhttps://genomebiology.biomedcentral.com/track/pdf/10.1186/s13059-019-1881-2
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleCRISPR-Cas13d mediates robust RNA virus interference in plants.
dc.typeArticle
dc.contributor.departmentBioscience Program
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentPlant Science
dc.contributor.departmentDesert Agriculture Initiative
dc.identifier.journalGenome biology
dc.eprint.versionPublisher's Version/PDF
kaust.personMahas, Ahmed
kaust.personAman, Rashid
kaust.personMahfouz, Magdy M.
refterms.dateFOA2019-12-12T07:05:21Z
display.relations<b> Is Supplemented By:</b> <br/> <ul> <li><i>[Dataset]</i> <br/> . DOI: <a href="https://doi.org/10.6084/m9.figshare.c.4766735">10.6084/m9.figshare.c.4766735</a> HANDLE: <a href="http://hdl.handle.net/10754/665165">10754/665165</a></li></ul>
dc.date.published-online2019-12-02
dc.date.published-print2019-12


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This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Except where otherwise noted, this item's license is described as This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.