CATION-CHLORIDE CO-TRANSPORTER 1 (CCC1) mediates plant resistance against Pseudomonas syringae.
Type
ArticleAuthors
Han, BaodaJiang, Yunhe
Cui, Guoxin

Mi, Jianing

Roelfsema, Rob
Mouille, Grégory
Sechet, Julien
Al-Babili, Salim

Aranda, Manuel

Hirt, Heribert
KAUST Department
Biological and Environmental Science and Engineering (BESE) DivisionCenter for Desert Agriculture
Marine Science Program
Plant Science
Red Sea Research Center (RSRC)
Date
2019-12-05Online Publication Date
2019-12-05Print Publication Date
2020-02Permanent link to this record
http://hdl.handle.net/10754/660531
Metadata
Show full item recordAbstract
Plasma membrane (PM) depolarization functions as an initial step in plant defense signaling pathways. However, only a few ion channels/transporters have been characterized in the context of plant immunity. Here, we show that the Arabidopsis (Arabidopsis thaliana) Na+:K+:2Cl- (NKCC) cotransporter CCC1 has a dual function in plant immunity. CCC1 functions independently of PM depolarization and negatively regulates pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI). However, CCC1 positively regulates plant basal and effector-triggered resistance to Pseudomonas syringae pv. tomato (Pst) DC3000. In line with the compromised immunity to Pst DC3000, ccc1 mutants show reduced expression of genes encoding enzymes involved in the biosynthesis of antimicrobial peptides, camalexin, and 4-OH-ICN, as well as Pathogenesis-Related (PR) proteins. Moreover, genes involved in cell wall and cuticle biosynthesis are constitutively downregulated in ccc1 mutants, and the cell walls of these mutants exhibit major changes in monosaccharide composition. The role of CCC1 ion transporter activity in the regulation of plant immunity is corroborated by experiments using the specific NKCC inhibitor bumetanide. These results reveal a function for ion transporters in immunity-related cell wall fortification and antimicrobial biosynthesis.Citation
Han, B., Jiang, Y., Cui, G., Mi, J., Roelfsema, R., Mouille, G., … Hirt, H. (2019). CATION-CHLORIDE CO-TRANSPORTER 1 (CCC1) mediates plant resistance against Pseudomonas syringae. Plant Physiology, pp.01279.2019. doi:10.1104/pp.19.01279Journal
Plant physiologyAdditional Links
http://www.plantphysiol.org/lookup/doi/10.1104/pp.19.01279ae974a485f413a2113503eed53cd6c53
10.1104/pp.19.01279