Show simple item record

dc.contributor.authorVezzoli, E
dc.contributor.authorCali, Corrado
dc.contributor.authorDe Roo, M
dc.contributor.authorPonzoni, L
dc.contributor.authorSogne, Elisa
dc.contributor.authorGagnon, N
dc.contributor.authorFrancolini, M
dc.contributor.authorBraida, D
dc.contributor.authorSala, M
dc.contributor.authorMuller, D
dc.contributor.authorFalqui, Andrea
dc.contributor.authorMagistretti, Pierre J.
dc.date.accessioned2019-12-11T12:35:48Z
dc.date.available2019-12-11T12:35:48Z
dc.date.issued2019-12-06
dc.identifier.citationVezzoli, E., Calì, C., De Roo, M., Ponzoni, L., Sogne, E., Gagnon, N., … Magistretti, P. J. (2019). Ultrastructural Evidence for a Role of Astrocytes and Glycogen-Derived Lactate in Learning-Dependent Synaptic Stabilization. Cerebral Cortex. doi:10.1093/cercor/bhz226
dc.identifier.doi10.1093/cercor/bhz226
dc.identifier.urihttp://hdl.handle.net/10754/660529
dc.description.abstractLong-term memory formation (LTM) is a process accompanied by energy-demanding structural changes at synapses and increased spine density. Concomitant increases in both spine volume and postsynaptic density (PSD) surface area have been suggested but never quantified in vivo by clear-cut experimental evidence. Using novel object recognition in mice as a learning task followed by 3D electron microscopy analysis, we demonstrate that LTM induced all aforementioned synaptic changes, together with an increase in the size of astrocytic glycogen granules, which are a source of lactate for neurons. The selective inhibition of glycogen metabolism in astrocytes impaired learning, affecting all the related synaptic changes. Intrahippocampal administration of l-lactate rescued the behavioral phenotype, along with spine density within 24 hours. Spine dynamics in hippocampal organotypic slices undergoing theta burst-induced long-term potentiation was similarly affected by inhibition of glycogen metabolism and rescued by l-lactate. These results suggest that learning primes astrocytic energy stores and signaling to sustain synaptic plasticity via l-lactate.
dc.publisherOxford University Press (OUP)
dc.relation.urlhttps://academic.oup.com/cercor/advance-article/doi/10.1093/cercor/bhz226/5625994
dc.relation.urlhttps://academic.oup.com/cercor/advance-article-pdf/doi/10.1093/cercor/bhz226/31233820/bhz226.pdf
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/ licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.titleUltrastructural Evidence for a Role of Astrocytes and Glycogen-Derived Lactate in Learning-Dependent Synaptic Stabilization.
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division, King Abdullah University of Science and Technology (KAUST), 23955-6900 Thuwal, Saudi Arabia.
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.identifier.journalCerebral cortex (New York, N.Y. : 1991)
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionDepartment of Basic Neuroscience, University of Geneva Medical School, 1206 Geneva, Switzerland.
dc.contributor.institutionDipartimento di Biotecnologie Mediche e Medicina Traslazionale, Università degli Studi di Milano, 20133 Milano, Italy.
dc.contributor.institutionCNR, Institute of Neuroscience, 20129 Milano, Italy.
kaust.personVezzoli, E
kaust.personCali, Corrado
kaust.personSogne, Elisa
kaust.personGagnon, N
kaust.personFalqui, Andrea
kaust.personMagistretti, Pierre J.
refterms.dateFOA2019-12-11T12:36:21Z
dc.date.published-online2019-12-06
dc.date.published-print2020-04-14


Files in this item

Thumbnail
Name:
Articlefile1.pdf
Size:
1.104Kb
Format:
PDF
Description:
Publisher's Version/PDF

This item appears in the following Collection(s)

Show simple item record

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/ licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.
Except where otherwise noted, this item's license is described as This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/ licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.