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dc.contributor.authorSurya, Sandeep Goud
dc.contributor.authorMajhi, Sanjit Manohar
dc.contributor.authorAgarwal, Dilip K
dc.contributor.authorLahcen, Abdellatif Ait
dc.contributor.authorYuvaraja, Saravanan
dc.contributor.authorChappanda, K. N.
dc.contributor.authorSalama, Khaled N.
dc.date.accessioned2019-12-10T06:02:03Z
dc.date.available2019-12-10T06:02:03Z
dc.date.issued2020
dc.identifier.citationSurya, S. G., Majhi, S. M., Agarwal, D. K., Lahcen, A. A., Yuvaraja, S., Chappanda, K. N., & Salama, K. N. (2019). A label-free aptasensor FET based on Au nanoparticle decorated Co3O4 nanorods and a SWCNT layer for detection of cardiac troponin T protein. Journal of Materials Chemistry B. doi:10.1039/c9tb01989h
dc.identifier.doi10.1039/c9tb01989h
dc.identifier.urihttp://hdl.handle.net/10754/660490
dc.description.abstractAcute myocardial infarction (AMI) is a serious health problem that must be identified in its early stages. Considerable progress has been made in understanding the condition of AMI through ascertaining the role of biomarkers, such as myoglobin, cardiac troponin proteins (T and I), creatine kinase-MB, and fatty acid-binding protein (FABP). A field-effect transistor (FET) is an effective platform; however, innovations are required in all layers of the FET for it to become robust and highly sensitive. For the first time, we made use of the synergistic combination of noble metal nanoparticles (AuNPs) with Co3O4 for the detection of cardiac troponin T (cTnT) in a FET platform. We determined the morphology of Au-decorated Co3O4 NRs and their electronic properties by characterizing the channel layer using electron microscopies and transient measurements. Subsequently, we performed the detection of cardiac troponin T by immobilizing its complementary biotinylated DNA aptamer on the channel surface using a drop-casting method. To understand the changes in drain current caused by this interaction, we probed our SWCNT-Co3O4 NR transistor with limited gate and drain bias (≤1 V), achieving a sensitivity of 0.5 μA μg-1 mL-1 for the Au-decorated NRs. A 250% increase in the sensitivity and a limit of detection (LOD) of 0.1 μg mL-1 were achieved by using this device. Finally, selectivity studies proved that this synergistic combination works well in the FET configuration for the successful detection of cTnT.
dc.description.sponsorshipWe thank Ulrich Buttner of Microfluidic Lab, part of the Nanofabrication Core Lab, King Abdullah University of Science and Technology (KAUST), Saudi Arabia for providing his assistance in the project.
dc.publisherRoyal Society of Chemistry (RSC)
dc.relation.urlhttp://xlink.rsc.org/?DOI=C9TB01989H
dc.relation.urlhttps://pubs.rsc.org/en/content/articlepdf/2019/tb/c9tb01989h
dc.rightsArchived with thanks to Journal of materials chemistry. B
dc.rights.urihttp://creativecommons.org/licenses/by-nc/3.0/
dc.titleA label-free aptasensor FET based on Au nanoparticle decorated Co3O4 nanorods and a SWCNT layer for detection of cardiac troponin T protein.
dc.typeArticle
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
dc.contributor.departmentSensors Lab, Advanced Membranes and Porous Materials Center, Computer, Electrical and Mathematical Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Saudi Arabia.
dc.contributor.departmentElectrical Engineering Program
dc.identifier.journalJournal of materials chemistry. B
dc.rights.embargodate2020-11-30
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionCRNTS, Indian Institute of Technology Bombay, Mumbai - 400076, India.
dc.contributor.institutionDepartment of Electrical and Electronics Engineering, Birla Institute of Technology and Science, Hyderabad 500078, India
kaust.personSurya, Sandeep Goud
kaust.personMajhi, Sanjit Manohar
kaust.personLahcen, Abdellatif Ait
kaust.personYuvaraja, Saravanan
kaust.personChappanda, Karumbaiah N
kaust.personSalama, Khaled N.
refterms.dateFOA2019-12-10T06:02:53Z
kaust.acknowledged.supportUnitNanofabrication Core Lab


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Archived with thanks to Journal of materials chemistry. B
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