A label-free aptasensor FET based on Au nanoparticle decorated Co3O4 nanorods and a SWCNT layer for detection of cardiac troponin T protein.
AuthorsSurya, Sandeep Goud
Majhi, Sanjit Manohar
Agarwal, Dilip K
Lahcen, Abdellatif Ait
Chappanda, K. N.
Salama, Khaled N.
KAUST DepartmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Sensors Lab, Advanced Membranes and Porous Materials Center, Computer, Electrical and Mathematical Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Saudi Arabia.
Electrical Engineering Program
Embargo End Date2020-11-30
Permanent link to this recordhttp://hdl.handle.net/10754/660490
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AbstractAcute myocardial infarction (AMI) is a serious health problem that must be identified in its early stages. Considerable progress has been made in understanding the condition of AMI through ascertaining the role of biomarkers, such as myoglobin, cardiac troponin proteins (T and I), creatine kinase-MB, and fatty acid-binding protein (FABP). A field-effect transistor (FET) is an effective platform; however, innovations are required in all layers of the FET for it to become robust and highly sensitive. For the first time, we made use of the synergistic combination of noble metal nanoparticles (AuNPs) with Co3O4 for the detection of cardiac troponin T (cTnT) in a FET platform. We determined the morphology of Au-decorated Co3O4 NRs and their electronic properties by characterizing the channel layer using electron microscopies and transient measurements. Subsequently, we performed the detection of cardiac troponin T by immobilizing its complementary biotinylated DNA aptamer on the channel surface using a drop-casting method. To understand the changes in drain current caused by this interaction, we probed our SWCNT-Co3O4 NR transistor with limited gate and drain bias (≤1 V), achieving a sensitivity of 0.5 μA μg-1 mL-1 for the Au-decorated NRs. A 250% increase in the sensitivity and a limit of detection (LOD) of 0.1 μg mL-1 were achieved by using this device. Finally, selectivity studies proved that this synergistic combination works well in the FET configuration for the successful detection of cTnT.
CitationSurya, S. G., Majhi, S. M., Agarwal, D. K., Lahcen, A. A., Yuvaraja, S., Chappanda, K. N., & Salama, K. N. (2019). A label-free aptasensor FET based on Au nanoparticle decorated Co3O4 nanorods and a SWCNT layer for detection of cardiac troponin T protein. Journal of Materials Chemistry B. doi:10.1039/c9tb01989h
SponsorsWe thank Ulrich Buttner of Microfluidic Lab, part of the Nanofabrication Core Lab, King Abdullah University of Science and Technology (KAUST), Saudi Arabia for providing his assistance in the project.
PublisherRoyal Society of Chemistry (RSC)
Except where otherwise noted, this item's license is described as Archived with thanks to Journal of materials chemistry. B