Anchorene is a carotenoid-derived regulatory metabolite required for anchor root formation in Arabidopsis
Dickinson, Alexandra J.
Xiao, Ting Ting
Kharbatia, Najeh M.
Benfey, Philip N.
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Chemical Science Program
Desert Agriculture Initiative
KAUST Catalysis Center (KCC)
Marine Science Program
Physical Science and Engineering (PSE) Division
Red Sea Research Center (RSRC)
Online Publication Date2019-11-27
Print Publication Date2019-11
Permanent link to this recordhttp://hdl.handle.net/10754/660474
MetadataShow full item record
AbstractAnchor roots (ANRs) arise at the root-shoot junction and are the least investigated type of <jats:italic>Arabidopsis</jats:italic> root. Here, we show that ANRs originate from pericycle cells in an auxin-dependent manner and a carotenogenic signal to emerge. By screening known and assumed carotenoid derivatives, we identified anchorene, a presumed carotenoid-derived dialdehyde (diapocarotenoid), as the specific signal needed for ANR formation. We demonstrate that anchorene is an <jats:italic>Arabidopsis</jats:italic> metabolite and that its exogenous application rescues the ANR phenotype in carotenoid-deficient plants and promotes the growth of normal seedlings. Nitrogen deficiency resulted in enhanced anchorene content and an increased number of ANRs, suggesting a role of this nutrient in determining anchorene content and ANR formation. Transcriptome analysis and treatment of auxin reporter lines indicate that anchorene triggers ANR formation by modulating auxin homeostasis. Together, our work reveals a growth regulator with potential application to agriculture and a new carotenoid-derived signaling molecule.
CitationJia, K.-P., Dickinson, A. J., Mi, J., Cui, G., Xiao, T. T., Kharbatia, N. M., … Al-Babili, S. (2019). Anchorene is a carotenoid-derived regulatory metabolite required for anchor root formation in Arabidopsis. Science Advances, 5(11), eaaw6787. doi:10.1126/sciadv.aaw6787
SponsorsWe thank B. Schaefer for valuable discussions and M. Khalid for technical support.
This work was supported by Baseline funding and the Research Grants Program-Round 4 (CRG4) from King Abdullah University of Science and Technology (KAUST) to S.A.-B., by the Arnold and Mabel Beckman Postdoctoral Fellowship to A.J.D., and by the Howard Hughes Medical Institute and the Gordon and Betty Moore Foundation (through grant GBMF3405) to P.N.B.
Except where otherwise noted, this item's license is described as This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.