Anchorene is a carotenoid-derived regulatory metabolite required for anchor root formation in Arabidopsis
Type
ArticleAuthors
Jia, Kunpeng
Dickinson, Alexandra J.
Mi, Jianing

Cui, Guoxin

Xiao, Ting Ting
Kharbatia, Najeh M.
Guo, Xiujie
Sugiono, Erli
Aranda, Manuel

Blilou, Ikram

Rueping, Magnus

Benfey, Philip N.
Al-Babili, Salim

KAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionChemical Science Program
Desert Agriculture Initiative
KAUST Catalysis Center (KCC)
Marine Science Program
Organics
Physical Science and Engineering (PSE) Division
Plant Science
Red Sea Research Center (RSRC)
Date
2019-11-27Online Publication Date
2019-11-27Print Publication Date
2019-11Permanent link to this record
http://hdl.handle.net/10754/660474
Metadata
Show full item recordAbstract
Anchor roots (ANRs) arise at the root-shoot junction and are the least investigated type of <jats:italic>Arabidopsis</jats:italic> root. Here, we show that ANRs originate from pericycle cells in an auxin-dependent manner and a carotenogenic signal to emerge. By screening known and assumed carotenoid derivatives, we identified anchorene, a presumed carotenoid-derived dialdehyde (diapocarotenoid), as the specific signal needed for ANR formation. We demonstrate that anchorene is an <jats:italic>Arabidopsis</jats:italic> metabolite and that its exogenous application rescues the ANR phenotype in carotenoid-deficient plants and promotes the growth of normal seedlings. Nitrogen deficiency resulted in enhanced anchorene content and an increased number of ANRs, suggesting a role of this nutrient in determining anchorene content and ANR formation. Transcriptome analysis and treatment of auxin reporter lines indicate that anchorene triggers ANR formation by modulating auxin homeostasis. Together, our work reveals a growth regulator with potential application to agriculture and a new carotenoid-derived signaling molecule.Citation
Jia, K.-P., Dickinson, A. J., Mi, J., Cui, G., Xiao, T. T., Kharbatia, N. M., … Al-Babili, S. (2019). Anchorene is a carotenoid-derived regulatory metabolite required for anchor root formation in Arabidopsis. Science Advances, 5(11), eaaw6787. doi:10.1126/sciadv.aaw6787Sponsors
We thank B. Schaefer for valuable discussions and M. Khalid for technical support.This work was supported by Baseline funding and the Research Grants Program-Round 4 (CRG4) from King Abdullah University of Science and Technology (KAUST) to S.A.-B., by the Arnold and Mabel Beckman Postdoctoral Fellowship to A.J.D., and by the Howard Hughes Medical Institute and the Gordon and Betty Moore Foundation (through grant GBMF3405) to P.N.B.
Journal
Science AdvancesAdditional Links
http://advances.sciencemag.org/lookup/doi/10.1126/sciadv.aaw6787https://advances.sciencemag.org/content/advances/5/11/eaaw6787.full.pdf
ae974a485f413a2113503eed53cd6c53
10.1126/sciadv.aaw6787
Scopus Count
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