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dc.contributor.authorDecataldo, Francesco
dc.contributor.authorDruet, Victor
dc.contributor.authorPappa, Anna-Maria
dc.contributor.authorTan, Ellasia
dc.contributor.authorSavva, Achilleas
dc.contributor.authorPitsalidis, C.
dc.contributor.authorInal, Sahika
dc.contributor.authorKim, Ji-Seon
dc.contributor.authorFraboni, Beatrice
dc.contributor.authorOwens, Roisin
dc.contributor.authorIandolo, D.
dc.date.accessioned2019-12-09T06:40:37Z
dc.date.available2019-12-09T06:40:37Z
dc.date.issued2019-12-17
dc.identifier.citationDecataldo, F., Druet, V., Pappa, A.-M., Tan, E., Savva, A., Pitsalidis, C., … Iandolo, D. (2019). BMP-2 functionalized PEDOT:PSS-based OECTs for stem cell osteogenic differentiation monitoring. Flexible and Printed Electronics. doi:10.1088/2058-8585/ab5bfc
dc.identifier.doi10.1088/2058-8585/ab5bfc
dc.identifier.urihttp://hdl.handle.net/10754/660466
dc.description.abstractStem cell osteogenic differentiation is a complex process, associated with a number of events such as the secretion of collagen type I, osteopontin, osteonectin, osteocalcin and Bone Morphogenic Protein 2 (BMP-2). These molecules can be used as markers to monitor stem cell fate while studying the effects of a specific osteogenic differentiation treatment (e.g. electrical stimulation). Currently available techniques, such as the evaluation of the expression levels of specific genes and end-point biochemical assays, do not allow real-time monitoring of cellular processes, therefore overlooking potentially interesting information. This study explores a promising functionalization strategy towards on-line electrical monitoring of stem cell osteogenic differentiation process, using an organic electrochemical transistor (OECT) to detect cytokines of interest, secreted by cells during the osteogenic differentiation process, such as BMP-2. In this work, antibodies against BMP-2 were anchored on the poly(3,4-ethylenedioxythiophene):polystyrene (PEDOT:PSS) sulfonate gate electrode of an OECT. The biofunctionalization process was evaluated using multiple techniques such as Atomic Force Microscopy, Electrochemical Raman Spectroscopy, Quartz crystal microbalance. Electrode properties were assessed by running chronoamperometric studies, as well as by characterizing the PEDOT:PSS thin film resistance to ion flow by electrochemical impedance spectroscopy and OECT performance using transient (AC) measurements. Finally, a proof-of-concept, biosensor measurement was performed to test our functionalization strategy for sensing, proving that the antibody-functionalized OECTs were able to detect recombinant BMP-2 at levels that are comparable to those used for in vitro stimulation of bone regeneration via soluble osteoinductive factors.
dc.description.sponsorshipD.I. would like to acknowledge the financial support by the H2020-MSCA-IF-2015 grant, “SMART-BONE” (GA No. 704175). D.I. and F.D. would like to thank Tobias Cramer for the fruitful discussions and insights. A.S. and S. I would like to acknowledge support from KAUST.
dc.publisherIOP Publishing
dc.relation.urlhttps://iopscience.iop.org/article/10.1088/2058-8585/ab5bfc
dc.rightsThis is an author-created, un-copyedited version of an article accepted for publication/published in Flexible and Printed Electronics. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at http://doi.org/10.1088/2058-8585/ab5bfc
dc.rights.urihttps://creativecommons.org/licences/by-nc-nd/3.0
dc.titleBMP-2 functionalized PEDOT:PSS-based OECTs for stem cell osteogenic differentiation monitoring.
dc.typeArticle
dc.contributor.departmentBiological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.identifier.journalFlexible and Printed Electronics
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionDepartment of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, United Kingdom.
dc.contributor.institutionDepartment of Physics and Astronomy, University of Bologna, Bologna, Italy.
dc.contributor.institutionDepartment of Physics and Centre for Plastic Electronics, Imperial College London, London, United Kingdom.
dc.contributor.institutionMines Saint-Etienne, University of Lyon, Université Jean Monnet, INSERM, Saint-Etienne France
kaust.personDruet, Victor
kaust.personSavva, Achilleas
kaust.personInal, Sahika
refterms.dateFOA2019-12-09T06:42:30Z


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This is an author-created, un-copyedited version of an article accepted for publication/published in Flexible and Printed Electronics. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at http://doi.org/10.1088/2058-8585/ab5bfc
Except where otherwise noted, this item's license is described as This is an author-created, un-copyedited version of an article accepted for publication/published in Flexible and Printed Electronics. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at http://doi.org/10.1088/2058-8585/ab5bfc