BMP-2 functionalized PEDOT:PSS-based OECTs for stem cell osteogenic differentiation monitoring.
KAUST DepartmentBiological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia
Biological and Environmental Sciences and Engineering (BESE) Division
Permanent link to this recordhttp://hdl.handle.net/10754/660466
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AbstractStem cell osteogenic differentiation is a complex process, associated with a number of events such as the secretion of collagen type I, osteopontin, osteonectin, osteocalcin and Bone Morphogenic Protein 2 (BMP-2). These molecules can be used as markers to monitor stem cell fate while studying the effects of a specific osteogenic differentiation treatment (e.g. electrical stimulation). Currently available techniques, such as the evaluation of the expression levels of specific genes and end-point biochemical assays, do not allow real-time monitoring of cellular processes, therefore overlooking potentially interesting information. This study explores a promising functionalization strategy towards on-line electrical monitoring of stem cell osteogenic differentiation process, using an organic electrochemical transistor (OECT) to detect cytokines of interest, secreted by cells during the osteogenic differentiation process, such as BMP-2. In this work, antibodies against BMP-2 were anchored on the poly(3,4-ethylenedioxythiophene):polystyrene (PEDOT:PSS) sulfonate gate electrode of an OECT. The biofunctionalization process was evaluated using multiple techniques such as Atomic Force Microscopy, Electrochemical Raman Spectroscopy, Quartz crystal microbalance. Electrode properties were assessed by running chronoamperometric studies, as well as by characterizing the PEDOT:PSS thin film resistance to ion flow by electrochemical impedance spectroscopy and OECT performance using transient (AC) measurements. Finally, a proof-of-concept, biosensor measurement was performed to test our functionalization strategy for sensing, proving that the antibody-functionalized OECTs were able to detect recombinant BMP-2 at levels that are comparable to those used for in vitro stimulation of bone regeneration via soluble osteoinductive factors.
CitationDecataldo, F., Druet, V., Pappa, A.-M., Tan, E., Savva, A., Pitsalidis, C., … Iandolo, D. (2019). BMP-2 functionalized PEDOT:PSS-based OECTs for stem cell osteogenic differentiation monitoring. Flexible and Printed Electronics. doi:10.1088/2058-8585/ab5bfc
SponsorsD.I. would like to acknowledge the financial support by the H2020-MSCA-IF-2015 grant, “SMART-BONE” (GA No. 704175). D.I. and F.D. would like to thank Tobias Cramer for the fruitful discussions and insights. A.S. and S. I would like to acknowledge support from KAUST.
JournalFlexible and Printed Electronics
Except where otherwise noted, this item's license is described as This is an author-created, un-copyedited version of an article accepted for publication/published in Flexible and Printed Electronics. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at http://doi.org/10.1088/2058-8585/ab5bfc