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dc.contributor.authorGómez-Gómez, Lourdes
dc.contributor.authorDiretto, Gianfranco
dc.contributor.authorAhrazem, Oussama
dc.contributor.authorAl-Babili, Salim
dc.date.accessioned2019-12-05T11:32:17Z
dc.date.available2019-12-05T11:32:17Z
dc.date.issued2019-11-20
dc.identifier.citationGómez-Gómez, L., Diretto, G., Ahrazem, O., & Al-Babili, S. (2019). Determination of In Vitro and In Vivo Activities of Plant Carotenoid Cleavage Oxygenases. Plant and Food Carotenoids, 63–74. doi:10.1007/978-1-4939-9952-1_5
dc.identifier.doi10.1007/978-1-4939-9952-1_5
dc.identifier.urihttp://hdl.handle.net/10754/660432
dc.description.abstractCarotenoid cleavage products, apocarotenoids, are biologically active compounds exerting important functions as chromophore, hormones, signaling molecules, volatiles, and pigments. Apocarotenoids are generally synthesized by the carotenoid cleavage dioxygenases (CCDs) that comprise a ubiquitous family of enzymes. The activity of plant CCDs was unraveled more than 20 years ago, with the characterization of the maize VP14, the first identified CCD. The protocol developed to determine the activity of this enzyme in vitro is still being used, with minor modifications. In addition, in vivo procedures have been developed during these years, mainly based on the exploitation of Escherichia coli cells engineered to produce specific carotenoid substrates. Further, technological developments have led to significant improvements, contributing to a more efficient detection of the reaction products. This chapter provides an updated set of detailed protocols suitable for the in vitro and in vivo characterization of the activities of CCDs, starting from well-established methods.
dc.publisherSpringer Nature
dc.relation.urlhttp://link.springer.com/10.1007/978-1-4939-9952-1_5
dc.rightsArchived with thanks to Methods in molecular biology (Clifton, N.J.)
dc.titleDetermination of In Vitro and In Vivo Activities of Plant Carotenoid Cleavage Oxygenases.
dc.typeBook Chapter
dc.contributor.departmentPlant Science
dc.contributor.departmentDesert Agriculture Initiative
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.identifier.journalMethods in molecular biology (Clifton, N.J.)
dc.rights.embargodate2020-11-21
dc.eprint.versionPost-print
dc.contributor.institutionDepartamento de Ciencia y Tecnología Agroforestal y Genética, Instituto Botánico, Universidad de Castilla-La Mancha, Albacete, Spain.
dc.contributor.institutionItalian National Agency for New Technologies, Energy, and Sustainable Development, Casaccia Research Centre, Rome, Italy.
kaust.personAl-Babili, Salim
refterms.dateFOA2020-11-21T00:00:00Z
dc.date.published-online2019-11-20
dc.date.published-print2020


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