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    A Method for Extraction and LC-MS-Based Identification of Carotenoid-Derived Dialdehydes in Plants.

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    Mi et al. 2019-accepted.pdf
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    Description:
    Accepted manuscript
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    Type
    Book Chapter
    Authors
    Mi, Jianing cc
    Jia, Kunpeng cc
    Balakrishna, Aparna
    Al-Babili, Salim cc
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Plant Science
    Desert Agriculture Initiative
    KAUST Grant Number
    CRG4
    Date
    2019-11-20
    Online Publication Date
    2019-11-20
    Print Publication Date
    2020
    Embargo End Date
    2020-11-21
    Permanent link to this record
    http://hdl.handle.net/10754/660431
    
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    Abstract
    We developed a chemical derivatization based ultra-high performance liquid chromatography-hybrid quadrupole-Orbitrap mass spectrometer (UHPLC-Q-Orbitrap MS) analytical method to identify low-abundant and instable carotenoid-derived dialdehydes (DIALs, diapocarotenoids) from plants. Application of this method enhances the MS response signal of DIALs, enabling the detection of diapocarotenoids, which is crucial for understanding the function of these compounds and for elucidating the carotenoid oxidative metabolic pathway in plants.
    Citation
    Mi, J., Jia, K.-P., Balakrishna, A., & Al-Babili, S. (2019). A Method for Extraction and LC-MS-Based Identification of Carotenoid-Derived Dialdehydes in Plants. Plant and Food Carotenoids, 177–188. doi:10.1007/978-1-4939-9952-1_13
    Sponsors
    This work was supported by base line funding and a Competitive Research Grant (CRG4) given to Salim Al-Babili from the King Abdullah University of Science and Technology (KAUST).
    Publisher
    Springer US
    Journal
    Methods in molecular biology (Clifton, N.J.)
    DOI
    10.1007/978-1-4939-9952-1_13
    Additional Links
    http://link.springer.com/10.1007/978-1-4939-9952-1_13
    ae974a485f413a2113503eed53cd6c53
    10.1007/978-1-4939-9952-1_13
    Scopus Count
    Collections
    Biological and Environmental Sciences and Engineering (BESE) Division; Book Chapters; Desert Agriculture Initiative

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