A Method for Extraction and LC-MS-Based Identification of Carotenoid-Derived Dialdehydes in Plants.

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Accepted manuscript
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2020-11-21
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Type
Book Chapter
Authors
Mi, Jianing cc
Jia, Kunpeng cc
Balakrishna, Aparna
Al-Babili, Salim cc
KAUST Department
Biological and Environmental Sciences and Engineering (BESE) Division
Plant Science
Desert Agriculture Initiative
KAUST Grant Number
CRG4
Date
2019-11-20
Online Publication Date
2019-11-20
Print Publication Date
2020
Embargo End Date
2020-11-21
Permanent link to this record
http://hdl.handle.net/10754/660431

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Abstract
We developed a chemical derivatization based ultra-high performance liquid chromatography-hybrid quadrupole-Orbitrap mass spectrometer (UHPLC-Q-Orbitrap MS) analytical method to identify low-abundant and instable carotenoid-derived dialdehydes (DIALs, diapocarotenoids) from plants. Application of this method enhances the MS response signal of DIALs, enabling the detection of diapocarotenoids, which is crucial for understanding the function of these compounds and for elucidating the carotenoid oxidative metabolic pathway in plants.
Citation
Mi, J., Jia, K.-P., Balakrishna, A., & Al-Babili, S. (2019). A Method for Extraction and LC-MS-Based Identification of Carotenoid-Derived Dialdehydes in Plants. Plant and Food Carotenoids, 177–188. doi:10.1007/978-1-4939-9952-1_13
Sponsors
This work was supported by base line funding and a Competitive Research Grant (CRG4) given to Salim Al-Babili from the King Abdullah University of Science and Technology (KAUST).
Publisher
Springer US
Journal
Methods in molecular biology (Clifton, N.J.)
DOI
10.1007/978-1-4939-9952-1_13
Additional Links
http://link.springer.com/10.1007/978-1-4939-9952-1_13
Collections
Biological and Environmental Sciences and Engineering (BESE) Division; Book Chapters; Desert Agriculture Initiative