A Method for Extraction and LC-MS-Based Identification of Carotenoid-Derived Dialdehydes in Plants.
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Desert Agriculture Initiative
KAUST Grant NumberCRG4
Online Publication Date2019-11-20
Print Publication Date2020
Embargo End Date2020-11-21
Permanent link to this recordhttp://hdl.handle.net/10754/660431
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AbstractWe developed a chemical derivatization based ultra-high performance liquid chromatography-hybrid quadrupole-Orbitrap mass spectrometer (UHPLC-Q-Orbitrap MS) analytical method to identify low-abundant and instable carotenoid-derived dialdehydes (DIALs, diapocarotenoids) from plants. Application of this method enhances the MS response signal of DIALs, enabling the detection of diapocarotenoids, which is crucial for understanding the function of these compounds and for elucidating the carotenoid oxidative metabolic pathway in plants.
CitationMi, J., Jia, K.-P., Balakrishna, A., & Al-Babili, S. (2019). A Method for Extraction and LC-MS-Based Identification of Carotenoid-Derived Dialdehydes in Plants. Plant and Food Carotenoids, 177–188. doi:10.1007/978-1-4939-9952-1_13
SponsorsThis work was supported by base line funding and a Competitive Research Grant (CRG4) given to Salim Al-Babili from the King Abdullah University of Science and Technology (KAUST).