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    Metagenomic characterization of bacterial and functional gene communities in reclaimed water distribution systems

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    Name:
    Changzhi Wang Thesis.pdf
    Size:
    1.782Mb
    Format:
    PDF
    Description:
    MS Thesis
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    Type
    Thesis
    Authors
    Wang, Changzhi cc
    Advisors
    Hong, Pei-Ying cc
    Committee members
    Pain, Arnab cc
    Daffonchio, Daniele cc
    Program
    Environmental Science and Engineering
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Date
    2019-11
    Embargo End Date
    2020-11-20
    Permanent link to this record
    http://hdl.handle.net/10754/660155
    
    Metadata
    Show full item record
    Access Restrictions
    At the time of archiving, the student author of this thesis opted to temporarily restrict access to it. The full text of this thesis will become available to the public after the expiration of the embargo on 2020-11-20.
    Abstract
    Water reuse is increasingly pursued to alleviate global water scarcity. However, wastewater treatment process does not achieve full removal of biological contaminants from wastewater, and hence some microorganisms and their genetic elements can be disseminated into the reclaimed water distribution systems (RWDS). A systematic identification and characterization of these biological contaminants is required. However, a broad characterization for large-scale data are limited. In this study, reclaimed water samples are investigated through metagenomic analyses to assess their bacterial and functional (metal resistance genes (MRGs); virulence factors (VFs)) communities at the entry and exit points of the RWDS. Furthermore, water quality data are investigated to evaluate the potential relationship with these metagenomic annotations. This study found that the organic carbon content was likely relevant to the increase of bacteria and functional genes in RWDS. It was also found that the variation of functional genes was not associated with their host, inferring the role of horizontal gene transfers or promiscuity of hosts for various functional genes. Furthermore, Pseudomonas was identified in one RWDS with significant increase at both bacterial and functional levels.
    Citation
    Wang, C. (2019). Metagenomic characterization of bacterial and functional gene communities in reclaimed water distribution systems. KAUST Research Repository. https://doi.org/10.25781/KAUST-64QRB
    DOI
    10.25781/KAUST-64QRB
    ae974a485f413a2113503eed53cd6c53
    10.25781/KAUST-64QRB
    Scopus Count
    Collections
    Biological and Environmental Sciences and Engineering (BESE) Division; Environmental Science and Engineering Program; Theses

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