Metagenomic characterization of bacterial and functional gene communities in reclaimed water distribution systems
KAUST DepartmentBiological and Environmental Science and Engineering (BESE) Division
Embargo End Date2020-11-20
Permanent link to this recordhttp://hdl.handle.net/10754/660155
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Access RestrictionsAt the time of archiving, the student author of this thesis opted to temporarily restrict access to it. The full text of this thesis will become available to the public after the expiration of the embargo on 2020-11-20.
AbstractWater reuse is increasingly pursued to alleviate global water scarcity. However, wastewater treatment process does not achieve full removal of biological contaminants from wastewater, and hence some microorganisms and their genetic elements can be disseminated into the reclaimed water distribution systems (RWDS). A systematic identification and characterization of these biological contaminants is required. However, a broad characterization for large-scale data are limited. In this study, reclaimed water samples are investigated through metagenomic analyses to assess their bacterial and functional (metal resistance genes (MRGs); virulence factors (VFs)) communities at the entry and exit points of the RWDS. Furthermore, water quality data are investigated to evaluate the potential relationship with these metagenomic annotations. This study found that the organic carbon content was likely relevant to the increase of bacteria and functional genes in RWDS. It was also found that the variation of functional genes was not associated with their host, inferring the role of horizontal gene transfers or promiscuity of hosts for various functional genes. Furthermore, Pseudomonas was identified in one RWDS with significant increase at both bacterial and functional levels.
CitationWang, C. (2019). Metagenomic characterization of bacterial and functional gene communities in reclaimed water distribution systems. KAUST Research Repository. https://doi.org/10.25781/KAUST-64QRB