Show simple item record

dc.contributor.authorHe, Yi
dc.contributor.authorXiong, Ling
dc.contributor.authorGao, Xuejuan
dc.contributor.authorHai, Miaomiao
dc.contributor.authorLiu, Yanping
dc.contributor.authorWang, Gao
dc.contributor.authorChen, Guo
dc.contributor.authorShuai, Jianwei
dc.contributor.authorJiao, Yang
dc.contributor.authorZhang, Xixiang
dc.contributor.authorLiu, Ruchuan
dc.contributor.authorLiu, Liyu
dc.date.accessioned2019-11-17T12:22:20Z
dc.date.available2019-11-17T12:22:20Z
dc.date.issued2019-10-28
dc.identifier.citationHe, Y., Xiong, L., Gao, X., Hai, M., Liu, Y., Wang, G., … Liu, L. (2020). Morphological quantification of proliferation-to-invasion transition in tumor spheroids. Biochimica et Biophysica Acta (BBA) - General Subjects, 1864(1), 129460. doi:10.1016/j.bbagen.2019.129460
dc.identifier.doi10.1016/j.bbagen.2019.129460
dc.identifier.urihttp://hdl.handle.net/10754/660059
dc.description.abstractBACKGROUND:Metastasis determines the lethality of cancer. In most clinical cases, patients are able to live with tumor proliferation before metastasis. Thus, the transition from tumor proliferation to metastasis/invasion is essential. However, the mechanism is still unclear and especially, the proliferation-to-metastasis/invasion transition point has not been well defined. Therefore, quantitative characterization of this transition is urgently needed. METHODS:We have successfully developed a home-built living-cell incubation system combined with an inverted optical microscope, and a systematic, quantitative approach to describing the major characteristic morphological parameters for the identification of the critical transition points for tumor-cell spheroids in a collagen fiber scaffold. RESULTS:The system focuses on in vitro tumor modeling, e.g. the development of tumor-cell spheroids in a collagen fiber scaffold and the monitoring of cell transition from proliferation to invasion. By applying this approach to multiple tumor spheroid models, such as U87 (glioma tumor), H1299 (lung cancer), and MDA-MB-231 (breast cancer) cells, we have obtained quantitative morphological references to evaluate the proliferation-to-invasion transition time, as well as differentiating the invasion potential of tumor cells upon environmental changes, i.e. drug application. CONCLUSIONS:Our quantitative approach provides a feasible clarification for the proliferation-to-invasion transition of in vitro tumor models (spheroids). Moreover, the transition time is a useful reference for the invasive potential of tumor cells. GENERAL SIGNIFICANCE:This quantitative approach is potentially applicable to primary tumor cells, and thus has potential applications in the fields of cancer metastasis investigations and clinical diagnostics.
dc.description.sponsorshipThis work was supported by the National Natural Science Foundation of China (Grant Nos. 11674043 and 11604030), the Fundamental Research Funds for the Central Universities (2018CDJDWL0011), and the Fundamental and Advanced Research Program of Chongqing (Grant #cstc2018jcyjAX0338), China. We have no conflict of interest.
dc.publisherElsevier BV
dc.relation.urlhttps://linkinghub.elsevier.com/retrieve/pii/S0304416519302466
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Biochimica et biophysica acta. General subjects. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biochimica et biophysica acta. General subjects, [[Volume], [Issue], (2019-11-02)] DOI: 10.1016/j.bbagen.2019.129460 . © 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titleMorphological quantification of proliferation-to-invasion transition in tumor spheroids.
dc.typeArticle
dc.contributor.departmentMaterial Science and Engineering Program
dc.contributor.departmentPhysical Science and Engineering (PSE) Division
dc.identifier.journalBiochimica et Biophysica Acta (BBA) - General Subjects
dc.rights.embargodate2020-11-02
dc.eprint.versionPost-print
dc.contributor.institutionChongqing Key Laboratory of Soft Condensed Matter Physics and Smart Materials, College of Physics, Chongqing University, Chongqing 401331, China.
dc.contributor.institutionDepartment of Physics, Xiamen University, Xiamen 361005, China.
dc.contributor.institutionMaterials Science and Engineering, Arizona State University, Tempe, AZ 85287, USA.
kaust.personZhang, Xixiang
refterms.dateFOA2020-12-13T08:04:52Z
dc.date.published-online2019-10-28
dc.date.published-print2020-01


Files in this item

Thumbnail
Name:
morphological classification of.pdf
Size:
4.497Mb
Format:
PDF
Description:
Accepted manuscript

This item appears in the following Collection(s)

Show simple item record